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JoVE Journal

Immunology and Infection

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Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

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10:17 min

October 9th, 2016

October 9th, 2016

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0:05

Title

1:15

Preparation of Biofilm Pellicles and Colonies

3:19

Ethanol Resistance Assay

5:17

Biofilm Colony Preparation for Scanning Electron Microscopy (SEM)

7:49

Results: Bacillus subtilis Pellicle and Biofilm Formation Is Altered in the Presence of the Small Molecule Inhibitor D-leucine

9:20

Conclusion

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Bacterial biofilms are important for human health, protecting their bacterial residents from environmental insults and anti-microbial agents. The goal of this procedure was to develop a model system to evaluate the effects of small molecule inhibi

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This study presents the development of reproducible methodologies to study biofilm inhibitors and their effects on Bacillus subtilis multicellularity.

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