Cardiomyocyte siRNA Transfection and Confocal Video Microscopy Analysis
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Results: Representative Cardiomyocyte Analyses
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Conclusion
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The overall goal of this procedure is to visualize cell cycle activity in postnatal cardiomyocytes and to determine their nuclearity. This method can help answer key questions in the cardiac regeneration field, such as does a cardiomyocyte really
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To distinguish cell division from cell cycle variations in cardiomyocytes, we present protocols using two transgenic mouse lines: Myh6-H2B-mCh transgenic mice, for the unequivocal identification of cardiomyocyte nuclei, and CAG-eGFP-anillin mice, for distinguishing cell division from cell cycle variations.