Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method

0:31

Introduction

2:32

Methodology: Solutions

2:51

Methodology: Cells Thawing and First Subculturing

4:33

Methodology: Verification of Confluency

5:21

Methodology: Second Subculturing, Cells Detaching and Counting

7:45

Methodology: Antibody Quantitation and Dilutions

9:37

Methodology: Neutralization Assay

13:00

Methodology: Spectrophotometric Analysis

13:54

Results: Dose Response Curve

15:23

Conclusion