Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method
9.2K Views
•
16:07 min
•
September 16th, 2017
DOI :
September 16th, 2017
•Chapters in this video
0:00
Title
0:31
Introduction
2:32
Methodology: Solutions
2:51
Methodology: Cells Thawing and First Subculturing
4:33
Methodology: Verification of Confluency
5:21
Methodology: Second Subculturing, Cells Detaching and Counting
7:45
Methodology: Antibody Quantitation and Dilutions
9:37
Methodology: Neutralization Assay
13:00
Methodology: Spectrophotometric Analysis
13:54
Results: Dose Response Curve
15:23
Conclusion
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