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DNA Methylation Analysis

Visión general

Methylation at CpG dinucleotides is a chemical modification of DNA hypothesized to play important roles in regulating gene expression. In particular, the methylation of clusters of methylation sites, called “CpG islands”, near promoters and other gene regulatory elements may contribute to the stable silencing of genes, for example, during epigenetic processes such as genomic imprinting and X-chromosome inactivation. At the same time, aberrant CpG methylation has been shown to be associated with cancer.

In this video, the biological functions and mechanisms of DNA methylation will be presented, along with various techniques used to identify methylation sites in the genome. We will then examine the steps of bisulfite analysis, one of the most commonly used methods for detecting DNA methylation, as well as several applications of this technique.

Procedimiento

DNA methylation is a chemical modification of DNA that affects gene expression under different cellular contexts. Many researchers are interested in the mechanism and functions of this process, as aberrant DNA methylation has been associated with diseases such as cancer.

In this video, we will cover the principles behind DNA methylation and methods to detect it. Then, we will explore a general protocol for one of these methods, bisulfite analysis, and some applications of this technique.

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Tags
DNA MethylationGene ExpressionCellular ContextsAberrant DNA MethylationCancerPrinciplesDetectBisulfite AnalysisApplicationsMethyl GroupCytosine BasesGuanine BasesCpGsUnmethylated CpGsCpG IslandsGene RegulationEpigenetic ProcessesGenomic ImprintingX chromosome Inactivation

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0:00

Overview

0:42

Principles of DNA Methylation

4:07

Generalized Procedure for Bisulfite Analysis

6:14

Applications

8:17

Summary

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