Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

DOI :

10.3791/55848-v

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January 10th, 2018

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14,096 Views

Kritika S. Katiyar^1,2,3*, Carla C. Winter^1,2,4*, Wisberty J. Gordián-Vélez^1,2,4, John C. O'Donnell^1,2, Yeri J. Song^1,5, Nicole S. Hernandez^1,5, Laura A. Struzyna^1,2,4, D. Kacy Cullen^1,2,5

¹Center for Brain Injury & Repair, Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, ²Center for Neurotrauma, Neurodegeneration & Restoration, Michael J. Crescenz Veterans Affairs Medical Center, ³School of Biomedical Engineering, Drexel University, ⁴Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, ⁵Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania

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We showcase the development of self-assembled, three-dimensional bundles of longitudinally aligned astrocytic somata and processes within a novel biomaterial encasement. These engineered "living scaffolds", exhibiting micron-scale diameter yet extending centimeters in length, may serve as test-beds to study neurodevelopmental mechanisms or facilitate neuroregeneration by directing neuronal migration and/or axonal pathfinding.

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