Results: Time-lapse Imaging to Track Mitochondrial Movement in Oligodendrocytes
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Conclusion
Transcripción
The overall goal of this viral transduction and time-lapse recording is to visualize movement of oligodendrocyte mitochondria in organotypic brain slices.This method can help answer key questions in the oligodendrocyte field.Such as how the oligod
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Myelinating oligodendrocytes promote rapid action potential propagation and neuronal survival. Described here is a protocol for oligodendrocyte-specific expression of fluorescent proteins in organotypic brain slices with subsequent time-lapse imaging. Further, a simple procedure for visualizing unstained myelin is presented.