Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

0:52

Recombinatorial Cloning to Generate Ready-for-injection Clones

2:23

Hydrodynamic Tail Vein Injection

4:22

Induction of Transfected CreER with Tamoxifen

5:33

Induction of Tetracycline-dependent Gene or shRNA Expression

6:35

Preparation of Mouse Liver for Analysis by Immunostaining

7:30

Results: Constitutive and Inducible Gene Expression in the Liver Following HTVI

8:47

Conclusion