Tissue Lysis, Sonication, and Antibody Preparation
2:52
Chromatin Immunoprecipitation
3:40
Washing and Reverse Cross-linking of Immunoprecipitated DNA-protein Complexes
4:43
Purification and Quantification of Precipitated DNA
6:00
Library Generation Using the NEBNext Ultra II DNA Library Preparation Kit
7:53
Results: Immunoprecipitation of Tumor Tissues and Cells Using High-throughput ChIP-sequencing
9:23
Conclusion
Transcribir
The overall goal of this protocol is to unbiasedly identify combinatorial chromatin state patterns in tumor tissues and cancer cell lines. This method could help answer key questions in the cancer epigenetics field such as how a barren histone mod
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Here, we describe an optimized high-throughput ChIP-sequencing protocol and computational analyses pipeline for the determination of genome-wide chromatin state patterns from frozen tumor tissues and cell lines.