Lipid Extraction from Animal Tissue and Methanolysis
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Quantification of Fatty Acids by GC-FID and 13C Analysis by Isotopologue Profiling
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Results: Fatty Acid 13C Isotopologue Profiling in Collembola
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Conclusion
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The overall goal of this isotopologue profiling is to follow the trophic transfer of dietary fatty acids in invertebrate consumers, including their processing and lipid metabolism, such as chain elongation or beta oxidation. This method can help a
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The fatty acid trophic marker approach, i.e., the assimilation of fatty acids as entire molecule and transfer into consumer tissue with no or minor modification, is hampered by knowledge gaps in fatty acid metabolism of small soil invertebrates. Isotopologue profiling is provided as a valuable tool to disentangle trophic interactions.