August 6th, 2018
•Protein-protein and protein-metabolite interactions are crucial for all cellular functions. Herein, we describe a protocol that allows parallel analysis of these interactions with a protein of choice. Our protocol was optimized for plant cell cultures and combines affinity purification with mass spectrometry-based protein and metabolite detection.
Tags
Videos relacionados
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados