JoVE Journal

Biochemistry

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2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

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Protein-protein and protein-metabolite interactions are crucial for all cellular functions. Herein, we describe a protocol that allows parallel analysis of these interactions with a protein of choice. Our protocol was optimized for plant cell cultures and combines affinity purification with mass spectrometry-based protein and metabolite detection.

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Title

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Preparation of Plant Cell Material

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Tandem Affinity Purification (TAP)

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Metabolite and Protein Extraction

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Results: Simultaneous Characterization of Protein-Protein and Protein-Metabolite Interactions Employing a Single-Step Tandem Affinity Purification Coupled with Mass Spectrometry

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Conclusion

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