A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
Monitoring Senescence by Senescence Associated ß-galactosidase (SA ß-gal) Staining
4:27
Quantifying ROS Induction during OIS
6:42
Quantifying IL-6 and IL-8 mRNA Expression for SASP Anaylsis using Real-time PCR
8:53
Quantifying the Levels of Secreted IL-6 and IL-8 Proteins for SASP Analysis using ELISA
10:56
Results: Representative ROS and SASP Expression Analysis during H-Ras-induced Senescence
12:25
Conclusion
Transcripción
The overall goal of this procedure is to provide sensitive techniques for monitoring intracellular ROS levels and senescence-associated secretory phenotype, or SASP, during cellular senescence. Here, we demonstrate that these methods successfully
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Intracellular ROS has been shown to play an important role in the induction of cellular senescence. Here, we describe a sensitive assay for quantifying ROS levels during cellular senescence. We also provide protocols for assessing the senescence-associated secretory phenotype, which reportedly contributes to various age-related dysfunctions.