Converting Fluorescence Intensity to the Mean Number of Binding Sites
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Results: Specific Binding of Fluorescent-Labeled Coagulation Factor X or fX to Artificial Phospholipid Vesicles
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Conclusion
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Using this quantity flow cytometry method researchers can calculate the kinetic and equilibrium customs of brought in membrane interaction and estimate the number of protein brought in by sites on the PPH membrane. The advantages of this method ar
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Here, we describe a set of methods for characterizing the interaction of proteins with membranes of cells or microvesicles.