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Moscow Institute of Physics and Technology

3 ARTICLES PUBLISHED IN JoVE

Biochemistry

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Mikhail Skliar^1,2, Vasiliy S. Chernyshev^3,4

¹Department of Chemical Engineering, University of Utah, ²The Nano Institute of Utah, University of Utah, ³Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, ⁴Biopharmaceutical Cluster 'Northern', Moscow Institute of Physics and Technology

A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties.

Immunology and Infection

Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung

Ivan V. Maslov¹, Andrey O. Bogorodskiy¹, Mariia V. Pavelchenko¹, Ilia O. Zykov¹, Natalya I. Troyanova², Valentin I. Borshchevskiy^1,3, Marina A. Shevchenko²

¹Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, ²Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ³Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich

We describe the method for quantitative analysis of the distribution of Aspergillus fumigatus conidia (3 µm in size) in the airways of mice. The method also can be used for the analysis of microparticles and nanoparticle agglomerate distribution in the airways in various pathological condition models.

Biochemistry

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

Nadezhda Podoplelova^1,2, Polina Soloveva^1,4, Andrei Garzon Dasgupta^1,2,3, Aleksandra Filkova^1,2, Mikhail Panteleev^1,2,3,4

¹Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, ²National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, ³Faculty of Physics, Lomonosov Moscow State University, ⁴Faculty of Biological and Medical Physics, Moscow Institute of Physics and Technology

Here, we describe a set of methods for characterizing the interaction of proteins with membranes of cells or microvesicles.