Enrico Gratton

Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy.

Interaction of apolipoprotein A-I in three different conformations with palmitoyl oleoyl phosphatidylcholine vesicles.

Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells.

A two-photon view of an enzyme at work: Crotalus atrox venom PLA2 interaction with single-lipid and mixed-lipid giant unilamellar vesicles.

Unfolding of pyridoxal 5'-phosphate-dependent O-acetylserine sulfhydrylase probed by time-resolved tryptophan fluorescence.

Different time evolution of oxyhemoglobin and deoxyhemoglobin concentration changes in the visual and motor cortices during functional stimulation: a near-infrared spectroscopy study.

Two-photon fluorescence lifetime imaging of the skin stratum corneum pH gradient.

NHE1 regulates the stratum corneum permeability barrier homeostasis. Microenvironment acidification assessed with fluorescence lifetime imaging.

Relationship between erythrocyte membrane phase properties and susceptibility to secretory phospholipase A2.

Cellular characterization of adenylate kinase and its isoform: two-photon excitation fluorescence imaging and fluorescence correlation spectroscopy.

Functional frequency-domain near-infrared spectroscopy detects fast neuronal signal in the motor cortex.

Camptothecin poly[n-(2-hydroxypropyl) methacrylamide] copolymers in antitopoisomerase-I tumor therapy: intratumor release and antitumor efficacy.

Detection of the fast neuronal signal on the motor cortex using functional frequency domain near infrared spectroscopy.

Mapping of hemodynamics on the human calf with near infrared spectroscopy and the influence of the adipose tissue thickness.

Giant vesicles, Laurdan, and two-photon fluorescence microscopy: evidence of lipid lateral separation in bilayers.

Fluorescence correlation spectroscopy.

Localized irregularities in hemoglobin flow and oxygenation in calf muscle in patients with peripheral vascular disease detected with near-infrared spectrophotometry.

Neonatal development of the stratum corneum pH gradient: localization and mechanisms leading to emergence of optimal barrier function.

Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods.

The roles of changes in deoxyhemoglobin concentration and regional cerebral blood volume in the fMRI BOLD signal.

High-pressure fluorescence correlation spectroscopy.

Absolute frequency-domain pulse oximetry of the brain: methodology and measurements.

Fast cerebral functional signal in the 100-ms range detected in the visual cortex by frequency-domain near-infrared spectrophotometry.

Visualizing lipid structure and raft domains in living cells with two-photon microscopy.

Distance measurement by circular scanning of the excitation beam in the two-photon microscope.

Noninvasive determination of the optical properties of adult brain: near-infrared spectroscopy approach.

Fluorescence-lifetime imaging techniques for microscopy.

Partitioning of dual-lipidated peptides into membrane microdomains: lipid sorting vs peptide aggregation.

Spatial-temporal studies of membrane dynamics: scanning fluorescence correlation spectroscopy (SFCS).

Partitioning of NaPi cotransporter in cholesterol-, sphingomyelin-, and glycosphingolipid-enriched membrane domains modulates NaPi protein diffusion, clustering, and activity.

Age-correlated changes in cerebral hemodynamics assessed by near-infrared spectroscopy.

Mitigating thermal mechanical damage potential during two-photon dermal imaging.

Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes.

3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells.

Visualization and analysis of apolipoprotein A-I interaction with binary phospholipid bilayers.

Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.

Measurement of brain activity by near-infrared light.

Interaction of human apolipoprotein A-I with model membranes exhibiting lipid domains.

Measuring fast dynamics in solutions and cells with a laser scanning microscope.

Lipid--protein interactions revealed by two-photon microscopy and fluorescence correlation spectroscopy.

Chromatin dynamics in interphase cells revealed by tracking in a two-photon excitation microscope.

Organelle transport along microtubules in Xenopus melanophores: evidence for cooperation between multiple motors.

Phosphoinositide specificity of and mechanism of lipid domain formation by annexin A2-p11 heterotetramer.

Melanosomes transported by myosin-V in Xenopus melanophores perform slow 35 nm steps.

Visualizing association of N-ras in lipid microdomains: influence of domain structure and interfacial adsorption.

Spectrally resolved neurophotonics: a case report of hemodynamics and vascular components in the mammalian brain.

Modulation of an optical needle's reflectivity alters the average photon path through scattering media.

3D particle tracking on a two-photon microscope.

Optical characterization of ultrasmall Si nanoparticles prepared through electrochemical dispersion of bulk Si.

Pressure tuning of the morphology of heterogeneous lipid vesicles: a two-photon-excitation fluorescence microscopy study.

Capillary electrophoresis of ultrasmall carboxylate functionalized silicon nanoparticles.

Sunscreen enhancement of UV-induced reactive oxygen species in the skin.

Effects of vasodilation on intrinsic optical signals in the mammalian brain: a phantom study.

Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.

Detection of cerebral ischemia in neurovascular surgery using quantitative frequency-domain near-infrared spectroscopy.

Intrinsic tumor biomarkers revealed by novel double-differential spectroscopic analysis of near-infrared spectra.

Interaction of high density lipoprotein particles with membranes containing cholesterol.

Effect of age on brain oxygenation regulation during changes in position.

Exploring dynamics in living cells by tracking single particles.

Phase fluctuations on the micron-submicron scale in GUVs composed of a binary lipid mixture.

The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis.

Regional differences of hemodynamics and oxygenation in the human calf muscle detected with near-infrared spectrophotometry.

Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode.

Fluorescence microscopy to study pressure between lipids in giant unilamellar vesicles.

The phasor approach to fluorescence lifetime imaging analysis.

Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy.

A novel fluorescence lifetime imaging system that optimizes photon efficiency.

Monomer dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies.

Image correlation spectroscopy of multiphoton images correlates with collagen mechanical properties.

Mapping the number of molecules and brightness in the laser scanning microscope.

Yeast ribosomal stalk heterogeneity in vivo shown by two-photon FCS and molecular brightness analysis.

Fluorescence studies of the interactions of ubiquinol-10 with liposomes.

Noninvasive determination of brain tissue oxygenation during sleep in obstructive sleep apnea: a near-infrared spectroscopic approach.

Genetically encoded probe for fluorescence lifetime imaging of CaMKII activity.

Chromatin dynamics during interphase explored by single-particle tracking.

Fluorescence correlation spectroscopy and photon counting histogram on membrane proteins: functional dynamics of the glycosylphosphatidylinositol-anchored urokinase plasminogen activator receptor.

Real-time multi-parameter spectroscopy and localization in three-dimensional single-particle tracking.

Analysis of molecular concentration and brightness from fluorescence fluctuation data with an electron multiplied CCD camera.

Spatial diffusivity and availability of intracellular calmodulin.

Endothelial adhesion receptors are recruited to adherent leukocytes by inclusion in preformed tetraspanin nanoplatforms.

Analysis of diffusion and binding in cells using the RICS approach.

Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method.

Stoichiometry of molecular complexes at adhesions in living cells.

One photon up, one photon down.

Intrinsic near-infrared spectroscopic markers of breast tumors.

Measuring diffusion of lipid-like probes in artificial and natural membranes by raster image correlation spectroscopy (RICS): use of a commercial laser-scanning microscope with analog detection.

Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes.

Spatiotemporal image correlation spectroscopy measurements of flow demonstrated in microfluidic channels.

Optimization of the signal-to-noise ratio of frequency-domain instrumentation for near-infrared spectro-imaging of the human brain.

Profilin interaction with phosphatidylinositol (4,5)-bisphosphate destabilizes the membrane of giant unilamellar vesicles.

Imaging barriers to diffusion by pair correlation functions.

Real-time nanomicroscopy via three-dimensional single-particle tracking.

Coherent movement of cell layers during wound healing by image correlation spectroscopy.

Molecular basis of the potent membrane-remodeling activity of the epsin 1 N-terminal homology domain.

Dynamics of lipid domain formation: fluctuation analysis.

Characterization of metabolic differences between benign and malignant tumors: high-spectral-resolution diffuse optical spectroscopy.

Lipid packing determines protein-membrane interactions: challenges for apolipoprotein A-I and high density lipoproteins.

In vivo imaging of single-molecule translocation through nuclear pore complexes by pair correlation functions.

Scanning laser image correlation for measurement of flow.

A two-step path to inclusion formation of huntingtin peptides revealed by number and brightness analysis.

Fluorescence correlation spectroscopy, raster image correlation spectroscopy, and number and brightness on a commercial confocal laser scanning microscope with analog detectors (Nikon C1).

In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow.

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

Raster image correlation spectroscopy in live cells.

Quantifying translational mobility in neurons: comparison between current optical techniques.

Major translocation of calcium upon epidermal barrier insult: imaging and quantification via FLIM/Fourier vector analysis.

Lessons in fluctuation correlation spectroscopy.

Non-invasive in vivo measurement of the tear film using spatial autocorrelation in a live mammal model.

Oligomerization state of dynamin 2 in cell membranes using TIRF and number and brightness analysis.

Cross-correlated fluctuation analysis reveals phosphorylation-regulated paxillin-FAK complexes in nascent adhesions.

Applied physics. Deeper tissue imaging with total detection.

Role of PDZK1 protein in apical membrane expression of renal sodium-coupled phosphate transporters.

Dynamic imaging of the sodium phosphate cotransporters.

Nanometer-scale optical imaging of collagen fibers using gold nanoparticles.

Enhancement of imaging depth in turbid media using a wide area detector.

Nanometer-scale imaging by the modulation tracking method.

Methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles.

Pre-corneal tear film thickness in humans measured with a novel technique.

Differential modulation of the molecular dynamics of the type IIa and IIc sodium phosphate cotransporters by parathyroid hormone.

Factors determining the superior performance of lipid/DNA/protammine nanoparticles over lipoplexes.

Concentration independent modulation of local micromechanics in a fibrin gel.

DC-SIGN and influenza hemagglutinin dynamics in plasma membrane microdomains are markedly different.

Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach.

Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue.

Fluorescence correlation spectroscopy of intact nuclear pore complexes.

Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy.

Correlation of functional and resting state connectivity of cerebral oxy-, deoxy-, and total hemoglobin concentration changes measured by near-infrared spectrophotometry.

3D nanometer images of biological fibers by directed motion of gold nanoparticles.

Breast cancer spatial heterogeneity in near-infrared spectra and the prediction of neoadjuvant chemotherapy response.

Impact of contralateral and ipsilateral reference tissue selection on self-referencing differential spectroscopy for breast cancer detection.

Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK.

Sucrose monoester micelles size determined by Fluorescence Correlation Spectroscopy (FCS).

Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D.

Spectroscopic properties of gold nanoparticles at the single-particle level in biological environments.

Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus.

Scanning image correlation spectroscopy.

Free extracellular diffusion creates the Dpp morphogen gradient of the Drosophila wing disc.

Measurement of distance with the nanoscale precise imaging by rapid beam oscillation method.

Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.

Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo.

Label-free separation of human embryonic stem cells and their differentiating progenies by phasor fluorescence lifetime microscopy.

Capturing directed molecular motion in the nuclear pore complex of live cells.

Investigation of Ebola VP40 assembly and oligomerization in live cells using number and brightness analysis.

Number and brightness analysis of LRRK2 oligomerization in live cells.

Inclined selective plane illumination microscopy adaptor for conventional microscopes.

NADH distribution in live progenitor stem cells by phasor-fluorescence lifetime image microscopy.

Metabolic trajectory of cellular differentiation in small intestine by Phasor Fluorescence Lifetime Microscopy of NADH.

NHE3 regulatory factor 1 (NHERF1) modulates intestinal sodium-dependent phosphate transporter (NaPi-2b) expression in apical microvilli.

Phasor-FLIM analysis of NADH distribution and localization in the nucleus of live progenitor myoblast cells.

Digital parallel frequency-domain spectroscopy for tissue imaging.

Phasor fluorescence lifetime microscopy of free and protein-bound NADH reveals neural stem cell differentiation potential.

Single-particle tracking demonstrates that actin coordinates the movement of the Ebola virus matrix protein.

Deep tissue fluorescence imaging and in vivo biological applications.

Phase synchronization of oxygenation waves in the frontal areas of children with attention-deficit hyperactivity disorder detected by optical diffusion spectroscopy correlates with medication.

Real-time analysis of metabolic activity within Lactobacillus acidophilus by phasor fluorescence lifetime imaging microscopy of NADH.

Two-photon excited fluorescence lifetime imaging and spectroscopy of melanins in vitro and in vivo.

Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM.

Tracking the mechanical dynamics of human embryonic stem cell chromatin.

Raster image correlation spectroscopy and number and brightness analysis.

A practical implementation of multifrequency widefield frequency-domain fluorescence lifetime imaging microscopy.

The Ebola virus matrix protein penetrates into the plasma membrane: a key step in viral protein 40 (VP40) oligomerization and viral egress.

Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells.

Shank2 contributes to the apical retention and intracellular redistribution of NaPiIIa in OK cells.

The role of cytoskeleton networks on lipid-mediated delivery of DNA.

IFITM proteins restrict viral membrane hemifusion.

Laurdan fluorescence lifetime discriminates cholesterol content from changes in fluidity in living cell membranes.

Fluid shear stress on endothelial cells modulates mechanical tension across VE-cadherin and PECAM-1.

Fast spatiotemporal correlation spectroscopy to determine protein lateral diffusion laws in live cell membranes.

Axial super resolution topography of focal adhesion by confocal microscopy.

Simplified confocal microscope for counting particles at low concentrations.

Cell matrix remodeling ability shown by image spatial correlation.

A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells.

Dual channel detection of ultra low concentration of bacteria in real time by scanning FCS.

Spectral Phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR.

Assessing tumor contrast in radiographically dense breast tissue using Diffuse Optical Spectroscopic Imaging (DOSI).

Prefrontal hemodynamic changes during cigarette smoking in young adult smokers with and without ADHD.

Measuring actin flow in 3D cell protrusions.

Organic nitrogen uptake by arbuscular mycorrhizal fungi in a boreal forest.

Detecting Pyronin Y labeled RNA transcripts in live cell microenvironments by phasor-FLIM analysis.

Fluctuation-based imaging of nuclear Rac1 activation by protein oligomerisation.

A deep tissue fluorescence imaging system with enhanced SHG detection capabilities.

Golgi sorting regulates organization and activity of GPI proteins at apical membranes.

Advanced fluorescence microscopy methods for the real-time study of transcription and chromatin dynamics.

Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer.

Laurdan Monitors Different Lipids Content in Eukaryotic Membrane During Embryonic Neural Development.

Nanoimaging of focal adhesion dynamics in 3D.

Chromatin dynamics during DNA repair revealed by pair correlation analysis of molecular flow in the nucleus.

Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga.

Integrin-associated complexes form hierarchically with variable stoichiometry in nascent adhesions.

Intracellular trafficking of cationic liposome-DNA complexes in living cells.