a-surgical-procedure-for-resecting-mouse-rib-model-for-large-scale

A Surgical Procedure for Resecting the Mouse Rib: A Model for Large-Scale Long Bone Repair

This protocol introduces researchers to a new model for large-scale bone repair utilizing the mouse rib. The procedure details the following: preparation ...

Keck School of Medicine, University of Southern California

Recent evidence suggests a survival advantage in trauma patients who receive controlled or hypotensive resuscitation volumes. This study examines the threshold crystalloid volume that is an independent risk factor for mortality after trauma.

Emergency department crystalloid resuscitation of 1.5 L or more is associated with increased mortality in elderly and nonelderly trauma patients.

Hyperglycemia after traumatic brain injury (TBI) is an independent predictor of mortality. Insulin deficiency, as opposed to elevated blood glucose, might be the reason for increased mortality. TBI patients with diabetes mellitus (DM) were analyzed to determine how insulin deficiency affects mortality after TBI.

Diabetic patients with traumatic brain injury: insulin deficiency is associated with increased mortality.

Underage drinking and driving is a national safety issue. We hypothesized students would have lenient thoughts and behaviors about drinking and driving. The aim of our study was to assess current adolescent behavior and attitudes on alcohol use and driving.

Attitudes and behaviors on alcohol use and impaired driving in adolescents.

The clinical need for methods to repair and regenerate large cartilage and bone lesions persists. One way to make new headway is to study skeletal regeneration when it occurs naturally. Cartilage repair is typically slow and incomplete. However, an exception to this observation can be found in the costal cartilages where complete repair has been reported in humans but the cellular and molecular mechanisms have not yet been characterized. In this study, we establish a novel animal model for cartilage repair using the mouse rib costal cartilage. We then use this model to test the hypothesis that the perichondrium, the dense connective tissue that surrounds the cartilage, is a tissue essential for repair. Our results show that full replacement of the resected cartilage occurs quickly (within 1-2 months) and properly differentiates, but that repair occurs only in the presence of the perichondrium. We then provide evidence that the rib perichondrium contains a special niche that houses chondrogenic progenitors that possess qualities particularly suited for mediating repair. Label-retaining cells can be found within the perichondrium that can give rise to new chondrocytes. Furthermore, the perichondrium proliferates and thickens during the healing period, and when ectopically placed can generate new cartilage. In conclusion, we have successfully established a model for hyaline cartilage repair in the mouse rib which should be useful for gaining a more detailed understanding of cartilage regeneration and ultimately for developing methods to improve cartilage and bone repair in other parts of the skeleton. © 2014 American Society for Bone and Mineral Research.

Marissa K. Srour

Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research

Keck School of Medicine, University of Southern California

A Surgical Procedure for Resecting the Mouse Rib: A Model for Large-Scale Long Bone Repair

Marissa K. Srour

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Keck School of Medicine, University of Southern California

A Surgical Procedure for Resecting the Mouse Rib: A Model for Large-Scale Long Bone Repair

Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research