isolation-and-cryopreservation-of-neonatal-rat-cardiomyocytes

Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes

Cell culture has become increasingly important in cardiac research, but due to the limited proliferation of cardiomyocytes, culturing cardiomyocytes is ...

College of Veterinary Medicine, North Carolina State University

University of North Carolina at Chapel Hill, North Carolina State University

Soochow University

If a fluorogenic compound, such as fluorescein diacetate, is added to a water solution containing living cells it becomes hydrolyzed by intracellular esterases into a fluorochrome whose fluorescence can be used to monitor the cytoplasmic pH and the cytoplasmic viscosity of the cells. In this paper we have used this technique to measure the effects of different concentrations of Co2+ and Cd2+ ions on the cytoplasmic pH and the cytoplasmic viscosity of a single cell culture. Our results indicate that the observed decrease in the efficiency of the intracellular hydrolyzation of fluorogenic substances in the presence of different concentrations of heavy metals could be caused by both a decrease in the cytoplasmic pH and an increase in the cytoplasmic viscosity. A decrease in cytoplasmic pH would decrease the effectiveness of the intracellular enzymes, whereas an increase in cytoplasmic viscosity would decrease diffusion which would also reduce the effectiveness of the reaction. The dependence of the reciprocal of the cytoplasmic viscosity on the concentration of these metals correlates well with published results on their toxicity.

Ke Cheng

Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research

College of Veterinary Medicine, North Carolina State University

Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes

Ke Cheng

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research

College of Veterinary Medicine, North Carolina State University

Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes

Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research