Nediljko Budisa

Congeneric lantibiotics from ribosomal in vivo peptide synthesis with noncanonical amino acids.

Recent advances in genetic code engineering in Escherichia coli.

Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code.

Conjugation of proteins by installing BIO-orthogonally reactive groups at their N-termini.

Expanded genetic code for the engineering of ribosomally synthetized and post-translationally modified peptide natural products (RiPPs).

Coupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins.

Fluorine-rich planetary environments as possible habitats for life.

Site-directed and global incorporation of orthogonal and isostructural noncanonical amino acids into the ribosomal lasso peptide capistruin.

Applying γ-substituted prolines in the foldon peptide: polarity contradicts preorganization.

γ-(S)-Trifluoromethyl proline: evaluation as a structural substitute of proline for solid state (19)F-NMR peptide studies.

Orthogonal translation meets electron transfer: in vivo labeling of cytochrome c for probing local electric fields.

Orthogonal dual-modification of proteins for the engineering of multivalent protein scaffolds.

Cell-free expression with the toxic amino acid canavanine.

Chemical Evolution of a Bacterial Proteome.

Strategy for Enhancement of (13)C-Photo-CIDNP NMR Spectra by Exploiting Fractional (13)C-Labeling of Tryptophan.

Entropic Contribution of Elongation Factor P to Proline Positioning at the Catalytic Center of the Ribosome.

Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids.

Incorporation of Amino Acids with Long-Chain Terminal Olefins into Proteins.

cis-trans-Amide isomerism of the 3,4-dehydroproline residue, the 'unpuckered' proline.

Towards Biocontained Cell Factories: An Evolutionarily Adapted Escherichia coli Strain Produces a New-to-nature Bioactive Lantibiotic Containing Thienopyrrole-Alanine.

One-Pot Synthesis of Unprotected Anomeric Glycosyl Thiols in Water for Glycan Ligation Reactions with Highly Functionalized Sugars.

Design of S-Allylcysteine in Situ Production and Incorporation Based on a Novel Pyrrolysyl-tRNA Synthetase Variant.

Construction of a polyproline structure with hydrophobic exterior using octahydroindole-2-carboxylic acid.

Biocatalysis with Unnatural Amino Acids: Enzymology Meets Xenobiology.

Discovery and Investigation of Natural Editing Function against Artificial Amino Acids in Protein Translation.

Prospects of Incorporation of Non-canonical Amino Acids for the Chemical Diversification of Antimicrobial Peptides.

An expanded genetic code for probing the role of electrostatics in enzyme catalysis by vibrational Stark spectroscopy.

Coupling genetic code expansion and metabolic engineering for synthetic cells.

Xenobiology: State-of-the-Art, Ethics, and Philosophy of New-to-Nature Organisms.

Peptidyl-Prolyl Model Study: How Does the Electronic Effect Influence the Amide Bond Conformation?

Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code.

Synthetic alienation of microbial organisms by using genetic code engineering: Why and how?

Selective F-Labeling of Functionalized Carboxylic Acids with Difluoromethyl Diazomethane (CF HCHN ).

Amide rotation trajectories probed by symmetry.

Deciphering the Fluorine Code-The Many Hats Fluorine Wears in a Protein Environment.

Global substitution of hemeproteins with noncanonical amino acids in Escherichia coli with intact cofactor maturation machinery.

On universal coding events in protein biogenesis.

Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5' UTR of tumor-associated mRNAs.

Hydrolysis, polarity, and conformational impact of C-terminal partially fluorinated ethyl esters in peptide models.

Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins.

Exploring hydrophobicity limits of polyproline helix with oligomeric octahydroindole-2-carboxylic acid.

Transmembrane Polyproline Helix.

Expanding the Genetic Code of and to Incorporate Non-canonical Amino Acids for Production of Modified Lantibiotics.

Painting argyrins blue: Negishi cross-coupling for synthesis of deep-blue tryptophan analogue β-(1-azulenyl)-l alanine and its incorporation into argyrin C.

Long-Range Modulations of Electric Fields in Proteins.

Site-Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater.

Site-Specific Chemoselective Pyrrolysine Analogues Incorporation Using the Cell-Free Protein Synthesis System.

Alternative Biochemistries for Alien Life: Basic Concepts and Requirements for the Design of a Robust Biocontainment System in Genetic Isolation.

Promotion of the collagen triple helix in a hydrophobic environment.

In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation.

Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine.

Phage capsid nanoparticles with defined ligand arrangement block influenza virus entry.

Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and Expression.

An Engineered Escherichia coli Strain with Synthetic Metabolism for in-Cell Production of Translationally Active Methionine Derivatives.

Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids.

Courses Based on iGEM/BIOMOD Competitions Are the Ideal Format for Research-Based Learning of Xenobiology.

Multiomics Analysis Provides Insight into the Laboratory Evolution of toward the Metabolic Usage of Fluorinated Indoles.

Local Electric Field Changes during the Photoconversion of the Bathy Phytochrome Agp2.

Engineering Pyrrolysyl-tRNA Synthetase for the Incorporation of Non-Canonical Amino Acids with Smaller Side Chains.