Johan Hofkens

Probing the influence of O2 on photoinduced reversible electron transfer in perylenediimide-triphenylamine-based dendrimers by single-molecule spectroscopy.

Higher-excited-state photophysical pathways in multichromophoric systems revealed by single-molecule fluorescence spectroscopy.

Single-enzyme kinetics of CALB-catalyzed hydrolysis.

Stretched exponential decay and correlations in the catalytic activity of fluctuating single lipase molecules.

Electron transfer at the single-molecule level in a triphenylamine-perylene imide molecule.

Evidence for the isomerization and decarboxylation in the photoconversion of the red fluorescent protein DsRed.

Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa.

Probing intramolecular Förster resonance energy transfer in a naphthaleneimide-peryleneimide-terrylenediimide-based dendrimer by ensemble and single-molecule fluorescence spectroscopy.

Single molecule spectroscopy as a probe for dye-polymer interactions.

Charge transfer enhanced annihilation leading to deterministic single photon emission in rigid perylene end-capped polyphenylenes.

Direct measurement of the end-to-end distance of individual polyfluorene polymer chains.

In situ space- and time-resolved sorption kinetics of anionic dyes on individual LDH crystals.

Frans De Schryver: forty years of photochemistry and photophysics.

Characterizing the fluorescence intermittency and photobleaching kinetics of dye molecules immobilized on a glass surface.

Spatially resolved observation of crystal-face-dependent catalysis by single turnover counting.

Do enzymes sleep and work?

Solvent and pH dependent fluorescent properties of a dimethylaminostyryl borondipyrromethene dye in solution.

Photoconversion in the red fluorescent protein from the sea anemone Entacmaea quadricolor: is cis-trans isomerization involved?

Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching.

Synthesis and single enzyme activity of a clicked lipase-BSA hetero-dimer.

Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy.

Fluorescent perylene diimide rotaxanes: spectroscopic signatures of wheel-chromophore interactions.

Inhomogeneity of electron injection rates in dye-sensitized TiO2: comparison of the mesoporous film and single nanoparticle behavior.

Formation of vesicles in block copolymer-fluorinated surfactant complexes.

CT-CT annihilation in rigid perylene end-capped pentaphenylenes.

LEDGINs inhibit late stage HIV-1 replication by modulating integrase multimerization in the virions.

Origin of simultaneous donor-acceptor emission in single molecules of peryleneimide-terrylenediimide labeled polyphenylene dendrimers.

Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.

Single-molecule spectroscopic investigation of energy migration processes in cyclic porphyrin arrays.

Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants.

Ultrafast excited-state dynamics of the photoswitchable protein Dronpa.

Space- and time-resolved visualization of acid catalysis in ZSM-5 crystals by fluorescence microscopy.

Non-conjugated, phenyl assisted coupling in through bond electron transfer in a perylenemonoimide-triphenylamine system.

Singlet-singlet annihilation leading to a charge-transfer intermediate in chromophore-end-capped pentaphenylenes.

Fluorescence of single molecules in polymer films: sensitivity of blinking to local environment.

Single molecule fluorescence spectroscopy of pH sensitive oligonucleotide switches.

Visualization of membrane rafts using a perylene monoimide derivative and fluorescence lifetime imaging.

Single-molecule fluorescence spectroscopy in (bio)catalysis.

Singlet oxygen photosensitization by EGFP and its chromophore HBDI.

3D nanoscopy: bringing biological nanostructures into sharp focus.

A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants.

Complexation of lipofectamine and cholesterol-modified DNA sequences studied by single-molecule fluorescence techniques.

High-throughput fabrication of organic nanowire devices with preferential internal alignment and improved performance.

Dynamic disorder and stepwise deactivation in a chymotrypsin catalyzed hydrolysis reaction.

Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa.

Radical polymerization tracked by single molecule spectroscopy.

Static and dynamic bimolecular fluorescence quenching of porphyrin dendrimers in solution.

Excitation energy migration processes in cyclic porphyrin arrays probed by single molecule spectroscopy.

Diffusion of myelin oligodendrocyte glycoprotein in living OLN-93 cells investigated by raster-scanning image correlation spectroscopy (RICS).

Dark states in monomeric red fluorescent proteins studied by fluorescence correlation and single molecule spectroscopy.

Photoactivation of silver-exchanged zeolite A.

Architecture and spatial organization in a triple-species bacterial biofilm synergistically degrading the phenylurea herbicide linuron.

Water-soluble monofunctional perylene and terrylene dyes: powerful labels for single-enzyme tracking.

Nano-patterned layers of a grafted coumarinic chromophore.

Morphology of large ZSM-5 crystals unraveled by fluorescence microscopy.

Photoinduced electron-transfer in perylenediimide triphenylamine-based dendrimers: single photon timing and femtosecond transient absorption spectroscopy.

Probing dimerization and intraprotein fluorescence resonance energy transfer in a far-red fluorescent protein from the sea anemone Heteractis crispa.

Exploration of single molecule events in a haloperoxidase and its biomimic: localization of halogenation activity.

Relating pore structure to activity at the subcrystal level for ZSM-5: an electron backscattering diffraction and fluorescence microscopy study.

Second-harmonic generation in GFP-like proteins.

Control of surface plasmon localization via self-assembly of silver nanoparticles along silver nanowires.

Linking phospholipase mobility to activity by single-molecule wide-field microscopy.

Polymers and single molecule fluorescence spectroscopy, what can we learn?

Subdiffraction limited, remote excitation of surface enhanced Raman scattering.

Characterization of fluorescence in heat-treated silver-exchanged zeolites.

Fluorescence-based analysis of enzymes at the single-molecule level.

Measuring diffusion of lipid-like probes in artificial and natural membranes by raster image correlation spectroscopy (RICS): use of a commercial laser-scanning microscope with analog detection.

Monitoring the interaction of a single G-protein key binding site with rhodopsin disk membranes upon light activation.

Towards direct monitoring of discrete events in a catalytic cycle at the single molecule level.

The fabrication of a thin, circular polymer film based phase shaper for generating doughnut modes.

Orientational effects in the excitation and de-excitation of single molecules interacting with donut-mode laser beams.

Synthesis and photophysics of core-substituted naphthalene diimides: fluorophores for single molecule applications.

Focusing plasmons in nanoslits for surface-enhanced Raman scattering.

Super-resolution reactivity mapping of nanostructured catalyst particles.

Direct evidence of high spatial localization of hot spots in surface-enhanced Raman scattering.

High-resolution single-turnover mapping reveals intraparticle diffusion limitation in Ti-MCM-41-catalyzed epoxidation.

Unraveling excited-state dynamics in a polyfluorene-perylenediimide copolymer.

Improved method for counting DNA molecules on biofunctionalized nanoparticles.

Synthetic and natural photoswitches.

Higher resolution in localization microscopy by slower switching of a photochromic protein.

Optical encoding of silver zeolite microcarriers.

Spectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores.

In situ observation of the emission characteristics of zeolite-hosted silver species during heat treatment.

Data storage based on photochromic and photoconvertible fluorescent proteins.

Influence of lipid heterogeneity and phase behavior on phospholipase A2 action at the single molecule level.

Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters.

Response to mixed substrate feeds of the structure and activity of a linuron-degrading triple-species biofilm.

Direct patterning of oriented metal-organic framework crystals via control over crystallization kinetics in clear precursor solutions.

Bandgap opening in oxygen plasma-treated graphene.

Fluorescence micro(spectro)scopy as a tool to study catalytic materials in action.

The rylene colorant family--tailored nanoemitters for photonics research and applications.

The transcriptional co-activator LEDGF/p75 displays a dynamic scan-and-lock mechanism for chromatin tethering.

Single-molecule spectroscopy: caught in a trap.

Molecular organization of hydrophobic molecules and co-adsorbed water in SBA-15 ordered mesoporous silica material.

Optical mapping of DNA: single-molecule-based methods for mapping genomes.

Energy transfer pathways in a rylene-based Triad.

Metal-organic framework single crystals as photoactive matrices for the generation of metallic microstructures.

Transcriptomic analysis of phenanthrene degrading Sphingomonas biofilms exposed to environmentally relevant solute and matric stresses.

Local elongation of endothelial cell-anchored von Willebrand factor strings precedes ADAMTS13 protein-mediated proteolysis.

Morpholinecarbonyl-Rhodamine 110 based substrates for the determination of protease activity with accurate kinetic parameters.

Photocatalytic growth of dendritic silver nanostructures as SERS substrates.

Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications.

Dynamic disorder in single-enzyme experiments: facts and artifacts.

Mapping of surface-enhanced fluorescence on metal nanoparticles using super-resolution photoactivation localization microscopy.

Quantitative multicolor super-resolution microscopy reveals tetherin HIV-1 interaction.

Spectroscopic characterization of Venus at the single molecule level.

Excitation wavelength dependent surface enhanced Raman scattering of 4-aminothiophenol on gold nanorings.

Excitation polarization sensitivity of plasmon-mediated silver nanotriangle growth on a surface.

Spectroscopic properties, excitation, and electron transfer in an anionic water-soluble poly(fluorene-alt-phenylene)-perylenediimide copolymer.

The Escherichia coli GTPase ObgE modulates hydroxyl radical levels in response to DNA replication fork arrest.

Ensemble and single particle fluorimetric techniques in concerted action to study the diffusion and aggregation of the glycine receptor α3 isoforms in the cell plasma membrane.

Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection.

Unraveling the fluorescence features of individual corrole NH tautomers.

Exposure to solute stress affects genome-wide expression but not the polycyclic aromatic hydrocarbon-degrading activity of Sphingomonas sp. strain LH128 in biofilms.

Extended adding-doubling method for fluorescent applications.

Fluorescent oxygen sensitive microbead incorporation for measuring oxygen tension in cell aggregates.

Three-dimensional visualization of defects formed during the synthesis of metal-organic frameworks: a fluorescence microscopy study.

Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa.

Time-resolved single molecule fluorescence spectroscopy of an α-chymotrypsin catalyzed reaction.

Fluorescent proteins: shine on, you crazy diamond.

Revealing the excited-state dynamics of the fluorescent protein Dendra2.

Self-assembled organic microfibers for nonlinear optics.

Bacterial Obg proteins: GTPases at the nexus of protein and DNA synthesis.

Auto-production of biosurfactants reverses the coffee ring effect in a bacterial system.

Synthesis and in vitro evaluation of a PDT active BODIPY-NLS conjugate.

The quantity and quality of dissolved organic matter as supplementary carbon source impacts the pesticide-degrading activity of a triple-species bacterial biofilm.

Environmental dissolved organic matter governs biofilm formation and subsequent linuron degradation activity of a linuron-degrading bacterial consortium.

Determination of the bulk scattering parameters of diffusing materials.

Automatic particle detection in microscopy using temporal correlations.

Evaluation of the biofilm forming capacity of the 2, 6-dichlorobenzamide (BAM) degrading Aminobacter sp. strain MSH1 at macropollutant and micropollutant BAM concentrations.

Role of PFKFB3-driven glycolysis in vessel sprouting.

Iron(III)-based metal-organic frameworks as visible light photocatalysts.

Single molecule methods for the study of catalysis: from enzymes to heterogeneous catalysts.

Photoswitchable fluorescent proteins for superresolution fluorescence microscopy circumventing the diffraction limit of light.

Excited state dynamics of photoswitchable fluorescent protein Padron.

Analysis of alpha3 GlyR single particle tracking in the cell membrane.

X-ray irradiation-induced formation of luminescent silver clusters in nanoporous matrices.

Excited state dynamics of the photoconvertible fluorescent protein Kaede revealed by ultrafast spectroscopy.

Green-to-red photoconvertible Dronpa mutant for multimodal super-resolution fluorescence microscopy.

Biofilm formation of a bacterial consortium on linuron at micropollutant concentrations in continuous flow chambers and the impact of dissolved organic matter.

Cellular localization and dynamics of the Mrr type IV restriction endonuclease of Escherichia coli.

A surface-bound molecule that undergoes optically biased Brownian rotation.

Super-resolution optical DNA Mapping via DNA methyltransferase-directed click chemistry.

Delayed electron-hole pair recombination in iron(III)-oxo metal-organic frameworks.

Single particle tracking of ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type-1 repeats) molecules on endothelial von Willebrand factor strings.

Membrane distribution of the glycine receptor α3 studied by optical super-resolution microscopy.

Confocal imaging with a fluorescent bile acid analogue closely mimicking hepatic taurocholate disposition.

HIV virions as nanoscopic test tubes for probing oligomerization of the integrase enzyme.

Membrane remodeling processes induced by phospholipase action.

Shear-stress-induced conformational changes of von Willebrand factor in a water-glycerol mixture observed with single molecule microscopy.

Estimation of the effective phase function of bulk diffusing materials with the inverse adding-doubling method.

A causal relation between bioluminescence and oxygen to quantify the cell niche.

Live-cell SERS endoscopy using plasmonic nanowire waveguides.

The use of the adding-doubling method for the optical optimization of planar luminescent down shifting layers for solar cells.

Effect of microstructure on population growth parameters of Escherichia coli in gelatin-dextran systems.

Reporter cell activity within hydrogel constructs quantified from oxygen-independent bioluminescence.

Fabrication of silver nanoparticles with limited size distribution on TiO2 containing zeolites.

Thermocapillary fingering in surfactant-laden water droplets.

A hybrid tool for spectral ray tracing simulations of luminescent cascade systems.

Emission properties of oxyluciferin and its derivatives in water: revealing the nature of the emissive species in firefly bioluminescence.

Rationalizing inter- and intracrystal heterogeneities in dealuminated acid mordenite zeolites by stimulated Raman scattering microscopy correlated with super-resolution fluorescence microscopy.

Photophysical investigation of cyano-substituted terrylenediimide derivatives.

High-resolution single-molecule fluorescence imaging of zeolite aggregates within real-life fluid catalytic cracking particles.

Design and synthesis of nucleolipids as possible activated precursors for oligomer formation via intramolecular catalysis: stability study and supramolecular organization.

Combing of genomic DNA from droplets containing picograms of material.

The effect of medium structure complexity on the growth of Saccharomyces cerevisiae in gelatin-dextran systems.

Visualization of molecular fluorescence point spread functions via remote excitation switching fluorescence microscopy.

Radical C-H arylation of the BODIPY core with aryldiazonium salts: synthesis of highly fluorescent red-shifted dyes.

Diffraction-unlimited imaging: from pretty pictures to hard numbers.

Mapping pixel dissimilarity in wide-field super-resolution fluorescence microscopy.

Quantitative 3D Fluorescence Imaging of Single Catalytic Turnovers Reveals Spatiotemporal Gradients in Reactivity of Zeolite H-ZSM-5 Crystals upon Steaming.

Correction: A Causal Relation between Bioluminescence and Oxygen to Quantify the Cell Niche.

The pH-dependent photoluminescence of colloidal CdSe/ZnS quantum dots with different organic coatings.

Obg and Membrane Depolarization Are Part of a Microbial Bet-Hedging Strategy that Leads to Antibiotic Tolerance.

Accurate Diffusion Coefficients of Organosoluble Reference Dyes in Organic Media Measured by Dual-Focus Fluorescence Correlation Spectroscopy.

Radical C-H alkylation of BODIPY dyes using potassium trifluoroborates or boronic acids.

Molecular Dynamic Indicators of the Photoswitching Properties of Green Fluorescent Proteins.

Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM.

A study of SeqA subcellular localization in Escherichia coli using photo-activated localization microscopy.

Inhibition of Receptor Dimerization as a Novel Negative Feedback Mechanism of EGFR Signaling.

Ryanodine receptor cluster fragmentation and redistribution in persistent atrial fibrillation enhance calcium release.

Mechanism Behind the Apparent Large Stokes Shift in LSSmOrange Investigated by Time-Resolved Spectroscopy.

Resolving Interparticle Heterogeneities in Composition and Hydrogenation Performance between Individual Supported Silver on Silica Catalysts.

How Is cis-trans Isomerization Controlled in Dronpa Mutants? A Replica Exchange Molecular Dynamics Study.

Nanoscale Study of Polymer Dynamics.

Degradation of Methylammonium Lead Iodide Perovskite Structures through Light and Electron Beam Driven Ion Migration.

Super-resolution Localization and Defocused Fluorescence Microscopy on Resonantly Coupled Single-Molecule, Single-Nanorod Hybrids.

Electrochemistry: Photocatalysts in close-up.

Synthesis, Ensemble, and Single Molecule Characterization of a Diphenyl-Acetylene Linked Terrylenediimide Dimer.

Corrigendum: Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM.

Tuning the energetics and tailoring the optical properties of silver clusters confined in zeolites.

Decorating the Edges of a 2D Polymer with a Fluorescence Label.

Direct Observation of Luminescent Silver Clusters Confined in Faujasite Zeolites.

Simple microfluidic stagnation point flow geometries.

Surface Colonization and Activity of the 2,6-Dichlorobenzamide (BAM) Degrading Aminobacter sp. Strain MSH1 at Macro- and Micropollutant BAM Concentrations.

SOFI Simulation Tool: A Software Package for Simulating and Testing Super-Resolution Optical Fluctuation Imaging.

Photoconvertible Behavior of LSSmOrange Applicable for Single Emission Band Optical Highlighting.

Photoluminescence Blinking of Single-Crystal Methylammonium Lead Iodide Perovskite Nanorods Induced by Surface Traps.

Measuring the Viscosity of the Escherichia coli Plasma Membrane Using Molecular Rotors.

Single Molecule Nanospectroscopy Visualizes Proton-Transfer Processes within a Zeolite Crystal.