analysis-of-n-glycans-from-raphanus-sativus-cultivars-using-pngase-h

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

In recent years, the carbohydrate moieties of plants have received considerable attention, as they are a potential source of cross-reactive, ...

Nanjing Agricultural University

Health differences between breast- and formula-fed infants have long been apparent despite great efforts in improving the function of baby formula by adjusting the levels of various milk nutritional components. However, the N-glycome, a type of oligosaccharide decorating a diverse range of proteins, has not been extensively studied in milk regarding its biological function. In this study, the anti-pathogenic function of the enzymatically released human and bovine milk N-glycome against 5 food-borne pathogens was investigated. The human milk N-glycome showed significantly higher activity than bovine milk. After enzymatic defucosylation of human and bovine N-glycan pool, UHPLC peak shifts were observed in both suggesting heavy fucosylation of samples. Furthermore, the anti-pathogenic activity of the defulosylated N-glycome decreased significantly, and the significance of functional difference between the two almost disappeared. This result indicates the essential role of fucosylation for the anti-pathogenic function of the milk N-glycome, especially in human milk.

Ya-Min Du

Glycomics and Glycan Bioengineering Research Center (GGBRC),
College of Food Science and Technology,
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology

Nanjing Agricultural University

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺

Ya-Min Du

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Nanjing Agricultural University

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Glycomics and Glycan Bioengineering Research Center (GGBRC),
College of Food Science and Technology,
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺