droplet-barcoding-based-single-cell-transcriptomics-adult-mammalian

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

The analysis of single cell gene expression across thousands of individual cells within a tissue or microenvironment is a valuable tool for identifying ...

University of Calgary

Randomized controlled clinical trials (CTs) are gold standard tools for assessing interventions. Although burn CTs have improved care, their status, publication frequency, and publication quality are not known.

Burn clinical trials: A systematic review of registration and publications.

Hair follicle regeneration is dependent on reciprocal signaling between epithelial cells and underlying mesenchymal cells within the dermal papilla. Hair follicle dermal stem cells reside within the hair follicle mesenchyme, self-renew in vivo, and function to repopulate the dermal papilla and regenerate the connective tissue sheath with each hair cycle. The identity and temporal pattern of signals that regulate hair follicle dermal stem cell function are not known. Here, we show that platelet-derived growth factor signaling is crucial for hair follicle dermal stem cell function and platelet-derived growth factor deficiency results in a progressive depletion of the hair follicle dermal stem cell pool and their progeny. Using  mice, we ablated  specifically within the adult hair follicle dermal stem cell lineage. This led to significant loss of hair follicle dermal stem cell progeny in connective tissue sheath and dermal papilla of individual follicles, and a progressive reduction in total number of anagen hair follicles containing YFP cells. As well, over successive hair cycles, fewer hair follicle dermal stem cells were retained within each telogen hair follicle suggesting an impact on hair follicle dermal stem cell self-renewal. To further assess this, we grew prospectively isolated hair follicle dermal stem cells (Sox2GFP αSMAdsRed) in the presence or absence of platelet-derived growth factor ligands. Platelet-derived growth factor-BB enhanced proliferation, increased the frequency of Sox2 hair follicle dermal stem cell progeny and improved inductive capacity of hair follicle dermal stem cells in an ex vivo hair follicle formation assay. Similar effects on proliferation were observed in adult human SKPs. Our findings impart novel insights into the signals that comprise the adult hair follicle dermal stem cell niche and suggest that platelet-derived growth factor signaling promotes self renewal, is essential to maintain the hair follicle dermal stem cell pool and ultimately their regenerative capacity within the hair follicle.

Wisoo Shin

Department of Comparative Biology and Experimental Medicine,
Faculty of Veterinary Medicine,
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine

University of Calgary

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Wisoo Shin

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Comparative Biology and Experimental Medicine,Faculty of Veterinary Medicine,Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine

University of Calgary

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Department of Comparative Biology and Experimental Medicine,
Faculty of Veterinary Medicine,
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine