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A Task for Assessing the Impact of a Partner on the Speed and Accuracy of Motor Performance in Rats

To our knowledge, no study has examined the effect of mere presence on accuracy of performance in animals. Therefore, we developed an experimental task to ...

Doshisha University

In this paper, we examined the role of the medial prefrontal cortex in temporal discrimination in three experiments using rats. Experiment 1 attempted to dissociate the roles of the medial precentral (PrCm) area from the prelimbic and infralimbic (PL-IL) area in temporal discrimination using fixed-interval (FI) schedule. The gradient of response rate distribution became more moderate by a lesion of the PrCm, but not by a lesion of the PL-IL. In experiment 2, the efflux of acetylcholine (ACh) in the PrCm area during temporal discrimination tasks was compared to that during non-temporal discrimination tasks. ACh efflux was not different between these two tasks. In experiment 3, microinjection of the anticholinergic drug scopolamine (10 microg) into the PrCm area made the gradient of response rate distribution moderate. These findings suggest that reduced activity of the ACh system within the PrCm area impairs the precision of temporal discrimination, even though enhancement of this system is not indispensable for performing temporal discrimination.

Medial prefrontal cortex and precision of temporal discrimination: a lesion, microinjection, and microdialysis study.

Glutamate - a forgotten target for interval timing.

Several studies have demonstrated that the hippocampal N-methyl-D-aspartate type glutamate receptors (NMDARs) are necessary for the acquisition but not the retention of spatial reference memory. In contrast, a few studies have shown that post-acquisition repetitive intraperitoneal injections of an NMDAR antagonist facilitate the retention of spatial reference memory in a radial maze task. In the present study, we investigated the role of hippocampal NMDARs in the retention of spatial reference memories in Morris water maze. In Experiment 1, 24 h after training (4 trials/day for 4 days), D-AP5 was chronically infused into the hippocampus of rats for 5 days. In the subsequent probe test (seven days after training), we found that rats infused with D-AP5 spent a significantly longer time in the target quadrant compared to chance level, whereas rats in the control group did not. In Experiment 2, D-AP5 was infused into the hippocampus 1 (immediate) or 7 (delayed) days after the training session. In the probe test, following the retention interval of 13 days, immediate infusion facilitated the performance in a manner similar to Experiment 1, whereas the delayed infusion did not. These findings suggest that hippocampal NMDARs play an important role in the deterioration of spatial reference memory.

Post-acquisition hippocampal NMDA receptor blockade sustains retention of spatial reference memory in Morris water maze.

While it has been shown that the blockade of N-methyl-d-aspartate type glutamate receptors (NMDARs) impairs memory acquisition, recent studies have reported that the post-acquisition administration of NMDAR antagonists suppresses spatial memory decay. These findings suggest that NMDARs are important not only for the acquisition of new memories but also for the decay of previously acquired memories. The present study investigated the contributions of specific NMDAR subunits to spatial memory decay using NVP-AAM077 (NVP), an NMDAR antagonist that preferentially binds to GluN2A subunits, and the selective GluN2B blocker Ro 25-6981 (Ro). Following Morris water maze training (four trials/day for four days), NVP and/or Ro were subchronically infused into the rat hippocampus for five days. Seven days after training, NVP-treated rats and NVP/Ro-treated rats explored the target area significantly more than the control and Ro-treated rats. These results demonstrate that post-acquisition treatment with NVP, but not Ro, suppresses the forgetting of previously acquired spatial memories. The NVP-treated rats more persistently explored the target area in the second test, which was conducted one day after the first, while the NVP/Ro-treated rats did not, which suggest that Ro treatment downregulates memory retention. In conclusion, the present results indicate that the NMDAR GluN2A and GluN2B subunits contribute to spatial memory deterioration and maintenance, respectively.

Post-acquisition hippocampal blockade of the NMDA receptor subunit GluN2A but not GluN2B sustains spatial reference memory retention.

In this study, we investigated: (1) the effect of fear on interval timing-time perception in the seconds-to-minutes range-and (2) the role of the insular cortex in the modulation of this effect. Rats were first trained on a temporal bisection task in which their response to a lever A was reinforced following a 2.00-s tone, whereas their response to a lever B was reinforced following an 8.00-s tone. After acquisition, the rats were also presented with intermediate-duration tones and pressed one of two levers to indicate whether tone duration was closer to 2.00 or 8.00s. Subsequently, the rats underwent differential fear conditioning in which one pitch tone (conditioned stimulus; CS+) was paired with an electric foot shock, while the other pitch tone (CS-) was presented alone. Either artificial cerebrospinal fluid (aCSF) or the GABA agonist muscimol was then infused into the rats' bilateral insular cortex before the animals were tested on the bisection task using the CS+and CS- tones. We found that in the rats infused with aCSF, the point of subjective equality (PSE) of the CS+ was higher than that for CS-, suggesting that the duration for CS+ was perceived to be shorter than that of CS-. However, muscimol eliminated the difference in PSE between CS+ and CS- by generalizing of the effect from CS+to the CS-. Taken together, our results show that normal activity in the insular cortex is involved in fear-induced modulation of interval timing.

Insular cortex inactivation generalizes fear-induced underestimation of interval timing in a temporal bisection task.

We examined interval timing - time perception in the seconds-to-minutes range - of the fear-inducing stimulus and the role of the amygdala in this phenomenon. Rats were initially trained to perform a temporal bisection task, in which their responses to levers A and B were reinforced following 2-s and 8-s tones, respectively. After acquisition, the rats were also presented with tones of intermediate durations and pressed one of the two levers to indicate whether the tone duration was closer to 2 or 8 s. Subsequently, the rats underwent differential fear conditioning, in which one frequency tone (conditioned stimulus; CS+) was paired with an electric foot shock, whereas another frequency tone (CS-) was presented alone. The rats were then infused with artificial cerebrospinal fluid (aCSF) or the GABA agonist muscimol into the bilateral basolateral amygdala (BLA) before performing the bisection task with CS+ and CS-. In rats infused with aCSF, the psychophysical function shifted rightward in CS+ relative to that in CS-. Moreover, the point of subjective equality of the CS+ was higher than that of CS-, suggesting that the duration of the fear -CS was perceived as shorter than that of the neutral CS. However, muscimol infusion into the BLA abolished this difference, suggesting that BLA inactivation suppresses the effect of the fear -CS. Our results demonstrate that normal BLA activity is essential for fear-induced underestimation of time.

Basolateral amygdala inactivation eliminates fear-induced underestimation of time in a temporal bisection task.

Many studies on humans and animals have shown that the mere presence of another individual or individuals accelerates the motor performance speed of the subject individual. However, it has not been well investigated whether the mere presence of another individual affects the accuracy of motor performance in animals. In this study, we developed a novel task (run-and-pull task) to simultaneously investigate both the speed and accuracy of motor performance in rats and examined the effect of the mere presence of another rat on the task performance of the subject rat. Rats were first trained in isolation to run a runway and then pull a lever on the terminal end of the runway. After training, the subject rats were required to perform the task in isolation (Single) or in front of a non-competitive confederate rat without direct interaction (Pair). The results showed that the latency to start running and to pull the lever were shorter and the accuracy of the lever-pull movement was lower in the Pair condition than in the Single condition. These findings suggest that the mere presence of another individual increased the speed and decreased the accuracy of the motor performance of rats.

Toshimichi Hata

Faculty of Psychology

Doshisha University

A Task for Assessing the Impact of a Partner on the Speed and Accuracy of Motor Performance in Rats