Hinako Suga

Stimulation of increases in intracellular calcium and prostaglandin E2 generation in Chinese hamster ovary cells expressing receptor-Galpha16 fusion proteins.

Ligand screening system using fusion proteins of G protein-coupled receptors with G protein alpha subunits.

Use of acetylcholine mustard to study allosteric interactions at the M(2) muscarinic receptor.

Investigating the interaction of McN-A-343 with the M2 muscarinic receptor using its nitrogen mustard derivative.

Mutagenesis of nucleophilic residues near the orthosteric binding pocket of M1 and M2 muscarinic receptors: effect on the binding of nitrogen mustard analogs of acetylcholine and McN-A-343.

Analysis of agonism and inverse agonism in functional assays with constitutive activity: estimation of orthosteric ligand affinity constants for active and inactive receptor states.

Analysis of functional responses at G protein-coupled receptors: estimation of relative affinity constants for the inactive receptor state.