an-house-built-light-emitting-diode-based-photodynamic-therapy-device

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

This paper describes a novel, simple, and low-cost device to perform in vitro photodynamic therapy (PDT) assays, named the PhotoACT. The device was built ...

Federal University of Parana

A promising strategy to overcome multidrug resistance is the use of inhibitors of ABC drug transporters. For this reason, we evaluated the polyoxovanadates (POVs) [V O ] (V ), [H V O (PO )] (V ), [V O Cl] (V ) and [V O I] (V ) as inhibitors of three major multidrug resistance-linked ABC transporters: P-glycoprotein (P-gp), ABCG2 and MRP1. All of the POVs selectively inhibited P-gp. V and V were the two most promising compounds, with IC values of transport inhibition of 25.4 and 22.7 µm, respectively. Both compounds inhibited P-gp ATPase activity, with the same IC value of 1.26 µm. V and V triggered different conformational changes in the P-gp protein with time-dependent inhibition, which was confirmed using the synthesized salt of V with rhodamine B, RhoB-V . The hydrophilic nature of POVs supports the hypothesis that these compounds target an unusual ligand-binding site, opening new possibilities in the development of potent modulators of ABC transporters.

Isadora da Silva Zanzarini

Graduate Program in Pharmaceutical Sciences,
Laboratory of Cancer Drug Resistance,
Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance

Federal University of Parana

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Isadora da Silva Zanzarini

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Graduate Program in Pharmaceutical Sciences,Laboratory of Cancer Drug Resistance,Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance

Federal University of Parana

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Graduate Program in Pharmaceutical Sciences,
Laboratory of Cancer Drug Resistance,
Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance