in-vitro-synthesis-native-fibrous-long-spacing-segmental-long-spacing

<em>In vitro</em> Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Collagen fibrils are present in the  extracellular matrix of animal tissue to provide structural scaffolding and  mechanical strength. These native ...

University of Toronto

Abbiamo precedentemente dimostrato che un&#39;etichetta secondaria oro-coniugato pu&ograve; essere utilizzata per ridurre il limite di rilevazione in un&#39;analisi basata sulla diffrazione da pi&ugrave; di 40-fold. Mostriamo ora che usando una combinazione di un&#39;etichetta secondaria coniugato con perossidasi e un substrato precipitante che il limite di rilevazione in un&#39;analisi basata sulla diffrazione pu&ograve; essere ridotto da pi&ugrave; di 1000-fold. La risposta all&#39;accessorio secondario era lineare per concentrazioni da 50 a 2000 pg/mL di antidigossina.

Un enzima-amplificato basati sulla diffrazione immunoassay.

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We have previously shown that a gold-conjugated secondary label can be used to reduce the limit of detection in a diffraction-based assay by more than 40-fold. We now show that by using a combination of a peroxidase-conjugated secondary label and a precipitating substrate the limit of detection in a diffraction-based assay can be reduced by more than 1000-fold. The response to secondary enhancement was linear for concentrations from 50 to 2000 pg/mL of antidigoxin.

An enzyme-amplified diffraction-based immunoassay.

Nous avons d&eacute;j&agrave; montr&eacute; qu&#39;une &eacute;tiquette secondaire conjugu&eacute; or peut &ecirc;tre utilis&eacute;e pour r&eacute;duire la limite de d&eacute;tection dans une analyse ax&eacute;e sur la diffraction par plus de 40 fois. Maintenant, nous montrent qu&#39;en utilisant une combinaison d&#39;une &eacute;tiquette secondaire coupl&eacute; &agrave; la peroxydase et un substrat pr&eacute;cipitant que la limite de d&eacute;tection dans un essai diffraction peut &ecirc;tre r&eacute;duite de plus de 1000 fois. La r&eacute;ponse &agrave; la mise en valeur secondaire &eacute;tait lin&eacute;aire pour les concentrations de 50 &agrave; 2000 pg/mL d&#39;antidigoxin.

Un test immunologique amplification enzymatique ax&eacute;e sur la diffraction.

Wir haben bereits gezeigt, dass ein Gold-konjugierten sekund&auml;ren Label verwendet werden kann, der Nachweisgrenze in einer Beugung-gegr&uuml;ndeten Probe von mehr als 40-fache zu reduzieren. Das zeigen wir jetzt mit eine Kombination aus einer sekund&auml;ren Peroxidase-konjugierten-Bezeichnung und ein beschleunigender Substrat, die Nachweisgrenze in einer Beugung-gegr&uuml;ndeten Probe mehr als Abstand reduziert werden kann. Als Reaktion auf sekund&auml;re Erweiterung wurden linear f&uuml;r Konzentrationen von 50 bis 2000 Pg/mL Antidigoxin.

Jane Betty Goh

Department of Chemistry,
Institute for Optical Sciences

University of Toronto

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Jane Betty Goh

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Chemistry,Institute for Optical Sciences

University of Toronto

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Department of Chemistry,
Institute for Optical Sciences