Christoph H. Borchers

Identification of components of protein complexes using a fluorescent photo-cross-linker and mass spectrometry.

Direct MALDI-MS/MS of phosphopeptides affinity-bound to immobilized metal ion affinity chromatography beads.

3' end processing of Drosophila melanogaster histone pre-mRNAs: requirement for phosphorylated Drosophila stem-loop binding protein and coevolution of the histone pre-mRNA processing system.

Pseudo-MS3 in a MALDI orthogonal quadrupole-time of flight mass spectrometer.

Effects of TCDD upon IkappaB and IKK subunits localized in microsomes by proteomics.

Phosphorylation of stem-loop binding protein (SLBP) on two threonines triggers degradation of SLBP, the sole cell cycle-regulated factor required for regulation of histone mRNA processing, at the end of S phase.

Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex.

Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.

DNA binding by yeast Mlh1 and Pms1: implications for DNA mismatch repair.

Matrix-assisted laser desorption/ionization directed nano-electrospray ionization tandem mass spectrometric analysis for protein identification.

Rapid and sensitive identification of epitope-containing peptides by direct matrix-assisted laser desorption/ionization tandem mass spectrometry of peptides affinity-bound to antibody beads.

Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells.

Development of a protein chip: a MS-based method for quantitation of protein expression and modification levels using an immunoaffinity approach.

Multi-kinase phosphorylation of the APC/C activator Cdh1 revealed by mass spectrometry.

Fourier transform ion cyclotron resonance MS reveals the presence of a water molecule in an enzyme transition-state analogue complex.

Electrospray ionization tandem mass spectrometry of model peptides reveals diagnostic fragment ions for protein ubiquitination.

Isotopically coded cleavable cross-linker for studying protein-protein interaction and protein complexes.

Identification of components of protein complexes.

Mass spectrometric determination of protein ubiquitination.

Phosphorylation of the RGS protein Sst2 by the MAP kinase Fus3 and use of Sst2 as a model to analyze determinants of substrate sequence specificity.

Improved protein identification through the use of unstained gels.

Phosphatase-directed phosphorylation-site determination: a synthesis of methods for the detection and identification of phosphopeptides.

Histone demethylation by a family of JmjC domain-containing proteins.

Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding.

Absolute quantitation of cancer-related proteins using an MS-based peptide chip.

Cohesin protein SMC1 represses the nuclear receptor CAR-mediated synergistic activation of a human P450 gene by xenobiotics.

Discovery of a small-molecule HIV-1 integrase inhibitor-binding site.

Photoaffinity labeling combined with mass spectrometric approaches as a tool for structural proteomics.

Combining fluorescence detection and mass spectrometric analysis for comprehensive and quantitative analysis of redox-sensitive cysteines in native membrane proteins.

Inhibition of APCCdh1 activity by Cdh1/Acm1/Bmh1 ternary complex formation.

2-methoxyestradiol inhibits the anaphase-promoting complex and protein translation in human breast cancer cells.

Direct interaction with filamins modulates the stability and plasma membrane expression of CFTR.

Identification of covalent modifications in P450 2E1 by 1,2-epoxy-3-butene in vitro.

A systems-biology analysis of feedback inhibition in the Sho1 osmotic-stress-response pathway.

Mitotic phosphorylation of the anaphase-promoting complex inhibitory subunit Mnd2 is necessary for efficient progression through meiosis i.

Protein arrays for biomarker discovery.

A surface-focused biotinylation procedure identifies the Yersinia pestis catalase KatY as a membrane-associated but non-surface-located protein.

Novel neutrophil-derived proteins in bronchoalveolar lavage fluid indicate an exaggerated inflammatory response in pediatric cystic fibrosis patients.

An immunoaffinity tandem mass spectrometry (iMALDI) assay for detection of Francisella tularensis.

Mass spectrometric determination of protein ubiquitination.

Free-flow electrophoresis for top-down proteomics by Fourier transform ion cyclotron resonance mass spectrometry.

Electron capture dissociation of electrosprayed protein ions for spatially resolved hydrogen exchange measurements.

Data Self-Recalibration and Mixture Mass Fingerprint Searching (DASER-MMF) to enhance protein identification within complex mixtures.

BiPS, a photocleavable, isotopically coded, fluorescent cross-linker for structural proteomics.

Towards high-throughput metabolomics using ultrahigh-field Fourier transform ion cyclotron resonance mass spectrometry.

Quantitative iTRAQ proteome and comparative transcriptome analysis of elicitor-induced Norway spruce (Picea abies) cells reveals elements of calcium signaling in the early conifer defense response.

A human proteome detection and quantitation project.

Top-down proteomics on a high-field Fourier transform ion cyclotron resonance mass spectrometer.

Multiple reaction monitoring-based, multiplexed, absolute quantitation of 45 proteins in human plasma.

A comparison of MS/MS-based, stable-isotope-labeled, quantitation performance on ESI-quadrupole TOF and MALDI-TOF/TOF mass spectrometers.

Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma.

Accurate quantitation of standard peptides used for quantitative proteomics.

Hydrogen/deuterium exchange mass spectrometry with top-down electron capture dissociation for characterizing structural transitions of a 17 kDa protein.

Targeted proteomics using selected reaction monitoring reveals the induction of specific terpene synthases in a multi-level study of methyl jasmonate-treated Norway spruce (Picea abies).

Use of a combination of isotopically coded cross-linkers and isotopically coded N-terminal modification reagents for selective identification of inter-peptide crosslinks.

ICC-CLASS: isotopically-coded cleavable crosslinking analysis software suite.

Metabolomics: towards understanding host-microbe interactions.

Towards the development of an immuno MALDI (iMALDI) mass spectrometry assay for the diagnosis of hypertension.

Top-down analysis of recombinant histone H3 and its methylated analogs by ESI/FT-ICR mass spectrometry.

Proteomic signatures in plasma during early acute renal allograft rejection.

Mass-spectrometry-based clinical proteomics--a review and prospective.

Assay development for the determination of phosphorylation stoichiometry using multiple reaction monitoring methods with and without phosphatase treatment: application to breast cancer signaling pathways.

An isotopically coded CID-cleavable biotinylated cross-linker for structural proteomics.

A quantitative study of the effects of chaotropic agents, surfactants, and solvents on the digestion efficiency of human plasma proteins by trypsin.

Crosslinking combined with mass spectrometry for structural proteomics.

Characterizing short-lived protein folding intermediates by top-down hydrogen exchange mass spectrometry.

Improving the biomarker pipeline.

Identification of Leishmania-specific protein phosphorylation sites by LC-ESI-MS/MS and comparative genomics analyses.

Mass spectrometry-based technologies for high-throughput metabolomics.

Development of an immuno tandem mass spectrometry (iMALDI) assay for EGFR diagnosis.

Technology development: an overview.

Effect of antibiotic treatment on the intestinal metabolome.

Impact of salmonella infection on host hormone metabolism revealed by metabolomics.

An integrated genomic, proteomic and biochemical analysis of (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis) genotypes that are resistant or susceptible to white pine weevil.

The Saccharomyces cerevisiae fermentation stress response protein Igd1p/Yfr017p regulates glycogen levels by inhibiting the glycogen debranching enzyme.

Mass spectrometric analysis and mutagenesis predict involvement of multiple cysteines in redox regulation of the skeletal muscle ryanodine receptor ion channel complex.

Conformer-specific hydrogen exchange analysis of Aβ(1-42) oligomers by top-down electron capture dissociation mass spectrometry.

The human proteome project: current state and future direction.

Metabolomics reveals phospholipids as important nutrient sources during Salmonella growth in bile in vitro and in vivo.

The deubiquitinase activity of the Salmonella pathogenicity island 2 effector, SseL, prevents accumulation of cellular lipid droplets.

Emerging mass spectrometry-based technologies for analyses of chromatin changes: analysis of histones and histone modifications.

High-flow multiplexed MRM-based analysis of proteins in human plasma without depletion or enrichment.

Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam principles).

Recommendations for Mass Spectrometry Data Quality Metrics for Open Access Data (Corollary to the Amsterdam Principles).

Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam principles).

MeCP2 binds to nucleosome free (linker DNA) regions and to H3K9/H3K27 methylated nucleosomes in the brain.

The use of multiplexed MRM for the discovery of biomarkers to differentiate iron-deficiency anemia from anemia of inflammation.

Dietary patterns and ethnicity are associated with distinct plasma proteomic groups.

Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam principles).

Multiplexed quantification of 63 proteins in human urine by multiple reaction monitoring-based mass spectrometry for discovery of potential bladder cancer biomarkers.

Quantitation of spatially-localized proteins in tissue samples using MALDI-MRM imaging.

Repression of Salmonella enterica phoP expression by small molecules from physiological bile.

Duplexed iMALDI for the detection of angiotensin I and angiotensin II.

Use of proteinase K nonspecific digestion for selective and comprehensive identification of interpeptide cross-links: application to prion proteins.

Structure and dynamics of small soluble Aβ(1-40) oligomers studied by top-down hydrogen exchange mass spectrometry.

Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia.

A role for ubiquitinylation and the cytosolic proteasome in turnover of mitochondrial uncoupling protein 1 (UCP1).

Comparison of standard- and nano-flow liquid chromatography platforms for MRM-based quantitation of putative plasma biomarker proteins.

MRM-based multiplexed quantitation of 67 putative cardiovascular disease biomarkers in human plasma.

Mass spectrometry-based structural proteomics.

Identification of two small regulatory RNAs linked to virulence in Brucella abortus 2308.

The effect of pre-analytical variability on the measurement of MRM-MS-based mid- to high-abundance plasma protein biomarkers and a panel of cytokines.

Multiple reaction monitoring (MRM): principles and application to coronary artery disease.

Association between the plasma proteome and serum ascorbic acid concentrations in humans.

Mass Spectrometry in High-Throughput Clinical Biomarker Assays: Multiple Reaction Monitoring.

Plasma proteomics can discriminate isolated early from dual responses in asthmatic individuals undergoing an allergen inhalation challenge.

Dithranol as a MALDI matrix for tissue imaging of lipids by Fourier transform ion cyclotron resonance mass spectrometry.

Glutathione S-transferase (GST) M1, but not GSTT1, genotype influences plasma proteomic profiles in Caucasian and East Asian young adults.

Using multiple structural proteomics approaches for the characterization of prion proteins.

Association between the plasma proteome and plasma α-tocopherol concentrations in humans.

Multiplexed quantitation of endogenous proteins in dried blood spots by multiple reaction monitoring-mass spectrometry.

Standardized protocols for quality control of MRM-based plasma proteomic workflows.

Top-down structural analysis of posttranslationally modified proteins by Fourier transform ion cyclotron resonance-MS with hydrogen/deuterium exchange and electron capture dissociation.

Plasma 25-Hydroxyvitamin D, Hormonal Contraceptive Use, and the Plasma Proteome in Caucasian, East Asian, and South Asian Young Adults.

Comprehensive analysis of oil sands processed water by direct-infusion Fourier-transform ion cyclotron resonance mass spectrometry with and without offline UHPLC sample prefractionation.

Metabolomic analysis of key central carbon metabolism carboxylic acids as their 3-nitrophenylhydrazones by UPLC/ESI-MS.

Absolute quantitation of proteins in human blood by multiplexed multiple reaction monitoring mass spectrometry.

Multiplexed MRM-based quantitation of candidate cancer biomarker proteins in undepleted and non-enriched human plasma.

Computational biomarker pipeline from discovery to clinical implementation: plasma proteomic biomarkers for cardiac transplantation.

Analysis of selected sugars and sugar phosphates in mouse heart tissue by reductive amination and liquid chromatography-electrospray ionization mass spectrometry.

Design, implementation and multisite evaluation of a system suitability protocol for the quantitative assessment of instrument performance in liquid chromatography-multiple reaction monitoring-MS (LC-MRM-MS).

Development of MRM-based assays for the absolute quantitation of plasma proteins.

Developing an iMALDI method.

Advances in multiplexed MRM-based protein biomarker quantitation toward clinical utility.

Comparison of proteins in whole blood and dried blood spot samples by LC/MS/MS.

Hydroxyflavones as a new family of matrices for MALDI tissue imaging.

Method and platform standardization in MRM-based quantitative plasma proteomics.

Model of the Mediator middle module based on protein cross-linking.

Metabonomics reveals drastic changes in anti-inflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease.

HIF1A reduces acute lung injury by optimizing carbohydrate metabolism in the alveolar epithelium.

Metabolic Signatures of Triatomine Vectors of Trypanosoma cruzi Unveiled by Metabolomics.

Targeted quantitation of CVD-linked plasma proteins for biomarker verification and validation.

Pre-analytical and analytical variability in absolute quantitative MRM-based plasma proteomic studies.