Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and StretchThaís Girão-Silva *1,2, Miriam Helena Fonseca-Alaniz *1, Luís Alberto Oliveira Dallan 1, Iuri Cordeiro Valãdao 1, Gustavo Henrique Oliveira da Rocha 1, José Eduardo Krieger 1, Ayumi Aurea Miyakawa 1
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg
We describe a protocol to isolate and culture human saphenous vein endothelial cells (hSVECs). We also provide detailed methods to produce shear stress and stretch to study mechanical stress in hSVECs.
