Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies AntigenTyler P. Graf *1, Kadryn Kadasia *2, Sarah Melhorn 1, Eliza Kessler 3, Haisong Yang 2, Tsvetelina Baryakova 1, Samantha Brady 2, Kevin J. McHugh 1,4
1Department of Bioengineering, Rice University, 2Particles for Humanity, 3IMPACT Technology Development, 4Department of Chemistry, Rice University
This method describes the encapsulation of the rabies antigen into biodegradable polymeric microparticles with structural and material properties that enable pulsatile release after a predetermined delay. Enzyme-linked immunosorbent assay (ELISA) assessment of the antigen retrieved from the particle core confirms the presence of intact trimeric rabies virus glycoprotein through particle fabrication.
