Unfractionated Bulk Culture of Mouse Skeletal Muscle to Recapitulate Niche and Stem Cell QuiescenceLouai Zaidan *1, Perla Geara *1, Matthew J. Borok *1, Léo Machado 1, Despoina Mademtzoglou 1, Philippos Mourikis 1, Frederic Relaix 1,2,3,4
1Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France, 2Ecole nationale vétérinaire d'Alfort, IMRB, F-94700 Maisons-Alfort, France, 3EFS, IMRB, F-94010 Creteil, France, 4AP-HP, Hopital Mondor, Service d'histologie, F-94010 Creteil, France
Skeletal muscle comprises multiple cell types, including resident stem cells, each with a special contribution to muscle homeostasis and regeneration. Here, the 2D culture of muscle stem cells and the muscle cell niche in an ex vivo setting that preserves many of the physiological, in vivo, and environmental characteristics are described.
