Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast ResearchYuefeng Li 1,2, Xiaofen Wu 1,2, Ping Hu 1,2
1Guangzhou Laboratory, 2The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University
Researchers new to the epigenetic field will find CUT&Tag a significantly easier alternative to ChIP assays. CUT&Tag has tremendously benefited the epigenetic studies on rare and primary cell populations, generating high-quality data from very few cells. This protocol describes performing H3K4me1 CUT&Tag assays on mouse myoblasts isolated from mouse hindlimb muscles.
