Polycarbonate Ultracentrifuge Tube Re-Use in Proteomic Analyses of Extracellular VesiclesRachel M. E. Cahalane *1,2, Mandy E. Turner *1, Cassandra L. Clift 1, Mark C. Blaser 1, Gabrielle Bogut 1, Sydney Levy 1, Taku Kasai 1, Tom A. P. Driedonks 3,4, Esther N. M. Nolte-‘t Hoen 4, Masanori Aikawa 1,5, Sasha A. Singh 1,5, Elena Aikawa 1,5
1Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, 2Mechanobiology and Medical Device Research Group (MMDRG), Biomedical Engineering, College of Science and Engineering, University of Galway, 3Department of CDL Research, University Medical Center Utrecht, 4Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, 5Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School
A detailed protocol is provided for cleaning and re-using polycarbonate ultracentrifuge tubes to perform extracellular vesicle isolation suitable for proteomics experiments.
