Functional Cloning Using a Xenopus Oocyte Expression SystemCarol Zygar Plautz 1, Hannah C. Williams 1, Robert M. Grainger 2
1Department of Biology, Shepherd University, 2Department of Biology, University of Virginia
We describe a Xenopus oocyte and animal cap system for the expression cloning of genes capable of inducing a response in competent ectoderm, and discuss techniques for the subsequent analysis of such genes. This system is useful in the functional identification of a wide range of gene products.
