S'identifier

Lawrence University

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Flexural Rigidity Measurements of Biopolymers Using Gliding Assays

Douglas S. Martin¹, Lu Yu¹, Brian L. Van Hoozen¹

¹Department of Physics, Lawrence University

A method to measure the persistence length or flexural rigidity of biopolymers is described. The method uses a kinesin-driven microtubule gliding assay to experimentally determine the persistence length of individual microtubules and is adaptable to actin-based gliding assays.

Biochemistry

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Mercedes Pardo¹, Daniel Bode¹, Lu Yu¹, Jyoti S. Choudhary¹

¹Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute

Here we present protocols for affinity purification of protein complexes and their separation by blue native PAGE, followed by protein correlation profiling using label free quantitative mass spectrometry. This method is useful to resolve interactomes into distinct protein complexes.