A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer ImmunotherapyLiang Hu 1, Robert Berahovich 1, Yanwei Huang 1, Shiming Zhang 1, Jinying Sun 1, Xianghong Liu 1, Hua Zhou 1, Shirley Xu 1, Haoqi Li 1, Vita Golubovskaya 1, Lijun Wu 1
1ProMab Biotechnologies
This protocol outlines a detailed procedure for generating transient chimeric antigen receptor (CAR) T cells using non-integrating mRNA for cancer immunotherapy and provides reliable methods for evaluating CAR-T cells and their cytotoxic function.
