Direct Cryosectioning of Drosophila Heads for Enhanced Brain Fluorescence Staining and ImmunostainingJohn Watson 1, Jonathan R. Roth 2, Girish C. Melkani 1,3
1Department of Pathology, Division of Molecular and Cellular Pathology, Heersink School of Medicine, The University of Alabama at Birmingham, 2Department of Neurobiology, Heersink School of Medicine, The University of Alabama at Birmingham, 3UAB Nathan Shock Center
This study presents a simplified protocol for tissue processing involving decapitation, fixation, cryosectioning, fluorescence staining, immunostaining, and imaging, which can be extended to confocal and multiphoton imaging. The method maintains efficacy comparable to complex dissections, bypassing the need for advanced motor skills. Quantitative image analysis provides extensive investigative potential.
