Paul Scherrer Institute
2 ARTICLES PUBLISHED IN JoVE
Chemistry
A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)
Mohamed Tarik 1, Debora Foppiano 1,2, Adrian Hess 1,3, Christian Ludwig 1,2
1Bioenergy and Catalysis Laboratory (LBK), Energy and Environment Research Division (ENE), Paul Scherrer Institute, 2Environmental Engineering Institute (IIE), School of Architecture, Civil and Environmental Engineering (ENAC), École; Polytechnique Fédérale de Lausanne (EPFL), 3Institute for Atmospheric and Climate Science ETH Zurich
In this work a practical guide is provided, describing the different steps to establish the coupling of SMPS and ICPMS systems, and how to use them. Three descriptive examples are presented.
Biochemistry
Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
Filip Pamula 1,2, Jonas Mühle 1, Alain Blanc 3, Rony Nehmé 4, Patricia C. Edwards 4, Christopher G. Tate 4, Ching-Ju Tsai 1
1Laboratory of Biomolecular Research, Paul Scherrer Institute, 2Department of Biology, ETH Zürich, 3Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, 4Laboratory of Molecular Biology, Medical Research Council
This report describes screening of different detergents for preparing the visual GPCR, rhodopsin, and its complex with mini-Go. Biochemical methods characterizing the quality of the complex at different stages during purification are demonstrated. This protocol can be generalized to other membrane protein complexes for their future structural studies.
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