Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)Carlos A. Lopez 1, George G. Daaboul 2, Sunmin Ahn 2, Alexander P. Reddington 1, Margo R. Monroe 2, Xirui Zhang 2, Rostem J. Irani 3, Chunxiao Yu 4,5, Caroline A. Genco 4,5, Marina Cretich 6, Marcella Chiari 6, Bennett B. Goldberg 1, John H. Connor 5, M. Selim Ünlü 1,2
1Department of Electrical and Computer Engineering, Boston University , 2Department of Biomedical Engineering, Boston University , 3Center for Advanced Genomics Technology, Boston University , 4Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, 5Department of Microbiology, Boston University School of Medicine, 6CNR (National Research Council), Istituto di Chimica del Riconoscimento Molecolare
Quantitative, high-throughput, real-time, and label-free biomolecular detection (DNA, protein, etc.) on SiO2 surfaces can be achieved using a simple interferometric technique which relies on LED illumination, minimal optical components, and a camera. The Interferometric Reflectance Imaging Sensor (IRIS) is inexpensive, simple to use, and amenable to microarray formats.
