In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic ProgenitorsChiara Greggio *1, Filippo De Franceschi *1, Manuel Figueiredo-Larsen *2, Anne Grapin-Botton 1,2
1Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Swiss Institute for Experimental Cancer Research, 2DanStem, University of Copenhagen
The three-dimensional culture method described in this protocol recapitulates pancreas development from dispersed embryonic mouse pancreas progenitors, including their substantial expansion, differentiation and morphogenesis into a branched organ. This method is amenable to imaging, functional interference and manipulation of the niche.
