S'identifier

University of Texas

4 ARTICLES PUBLISHED IN JoVE

Biology

Monitoring Plant Hormones During Stress Responses

Marie J. Engelberth¹, Jurgen Engelberth¹

¹Department of Biology, University of Texas

A simple method is provided that allows for the rapid extraction and analysis of multiple plant hormones from small tissue samples. The procedure uses vapor phase extraction as the solemn purification step. Samples are analyzed by GC/MS with chemical ionization that produces mainly (M+1)+ ions.

Medicine

Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs

Thais Federici¹, Carl V. Hurtig¹, Kentrell L. Burks¹, Jonathan P. Riley¹, Vibhor Krishna², Brandon A. Miller¹, Eric A. Sribnick¹, Joseph H. Miller³, Natalia Grin¹, Jason J. Lamanna^1,4,5, Nicholas M. Boulis¹

¹Department of Neurosurgery, Emory University, ²Department of Neuroscience, Medical University of South Carolina, ³Division of Neurosurgery, University of Alabama, Birmingham, ⁴Department of Biomedical Engineering, Georgia Institute of Technology , ⁵Department of Biomedical Engineering, Emory University

Short visual description of the surgical technique and device used for the delivery of (gene and cell) therapies into the spinal cord. The technique is demonstrated in the animal but is entirely translatable and currently being used for human application.

Behavior

Task Interruption and Resumption Paradigm for Testing the Activation and Pursuit of an Abstract Thinking Goal

Jae-Eun Namkoong¹, Marlone D. Henderson²

¹Managerial Sciences Department, University of Nevada, ²Department of Psychology, University of Texas

This protocol was designed to test the activation and pursuit of cognitive goals (e.g., an abstract thinking goal) using the task interruption and resumption paradigm. The protocol is suitable for cognitive goals that are automatically pursued once activated, as the distraction procedure prevents goal pursuit during the interruption period.

Neuroscience

Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin

Brandon A. Miller^1,2, Shelei Pan³, Peter H. Yang³, Catherine Wang¹, Amanda L. Trout¹, Dakota DeFreitas³, Sruthi Ramagiri³, Scott D. Olson², Jennifer M. Strahle^3,4,5

¹Department of Neurosurgery, University of Kentucky, ²Department of Pediatric Surgery, University of Texas, ³Department of Neurological Surgery, Washington University in St. Louis School of Medicine, ⁴Department of Orthopedic Surgery, Washington University in St. Louis School of Medicine, ⁵Department of Pediatrics, Washington University in St. Louis School of Medicine

We present a model of neonatal intraventricular hemorrhage using rat pups that mimics the pathology seen in humans.