S'identifier

US Army Research Laboratory

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Deborah A. Sarkes¹, Justin P. Jahnke¹, Dimitra N. Stratis-Cullum¹

¹Sensors and Electron Devices Directorate, US Army Research Laboratory

Biopanning bacterial display libraries is a proven technique for discovery of peptide affinity reagents, a robust alternative to antibodies. The semi-automated sorting method herein has streamlined biopanning to decrease the occurrence of false positives. Here we illustrate the thought process and techniques applied in evaluating candidates and minimizing downstream analysis.

Engineering

Processing of Bulk Nanocrystalline Metals at the US Army Research Laboratory

Vincent H. Hammond¹, Billy C. Hornbuckle¹, Anit K. Giri¹, Anthony J. Roberts¹, Thomas L. Luckenbaugh², Joseph M. Marsico³, Scott M. Grendahl¹, Kristopher A. Darling¹

¹Weapons and Materials Research Directorate, US Army Research Laboratory, ²Bowhead Total Enterprise Solutions, LLC, ³Oak Ridge Institute for Science and Education

This paper provides a brief overview of the ongoing efforts at the Army Research Laboratory on the processing of bulk nanocrystalline metals with an emphasis on the methodologies used for the production of the novel metal powders.