S'identifier

USDA-Agricultural Research Service

2 ARTICLES PUBLISHED IN JoVE

Medicine

Isolating and Using Sections of Bovine Mesenteric Artery and Vein as a Bioassay to Test for Vasoactivity in the Small Intestine

James L. Klotz¹, Adam J. Barnes¹

¹Forage-Animal Production Research Unit, USDA-Agricultural Research Service

The small intestine is frequently exposed to toxins that can influence blood flow and negatively impact nutrient absorption. Using a multimyograph and mesenteric artery and vein isolates, compounds or toxins of interest can be screened for vasoactivity.

Biology

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Michael J. Rothrock Jr.¹, Kelli L. Hiett², John Gamble³, Andrew C. Caudill⁴, Kellie M. Cicconi-Hogan¹, J. Gregory Caporaso⁵

¹Egg Safety and Quality Research Unit, USDA-Agricultural Research Service, ²Poultry Microbiological Safety and Processing Research Unit, USDA-Agricultural Research Service, ³Department of Biochemistry and Biophysics, Oregon State University, ⁴College of Public Health, University of Georgia, ⁵Department of Biological Sciences, Center for Microbial Genetics and Genomics, Northern Arizona University

A novel semi-automated hybrid DNA extraction method for use with environmental poultry production samples was developed and demonstrated improvements over a common mechanical and enzymatic extraction method in terms of the quantitative and qualitative estimates of the total bacterial communities.