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St. Luke's Roosevelt Medical Center

1 ARTICLES PUBLISHED IN JoVE

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Biology

Protein Transfection of Mouse Lung
Patrick Geraghty 1, Robert Foronjy 1
1Department of Medicine, St. Luke's Roosevelt Medical Center

Transgenic mice or viral vectors have been used to increase protein expression within the lung. However, these techniques are time-consuming, technically challenging and have off-target effects that can confound results. Our protein transfection protocol uses a lipid based transfection reagent and an ultrafine microsprayer to uniformly deliver active protein to lung cells.

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