Culturing Primary Rat Inner Medullary Collecting Duct CellsDörte Faust 1, Andrea Geelhaar 1, Beate Eisermann 1, Jenny Eichhorst 2, Burkhard Wiesner 2, Walter Rosenthal 1,3, Enno Klussmann 1
1Anchored Signalling, Max-Delbrück-Center for Molecular Medicine, 2Leibniz Institute for Molecular Pharmacology (FMP), 3Charité University Medicine Berlin
Arginine-vasopressin (AVP) controls fine-tuning of body water homeostasis through facilitating water reabsorption by renal principal cells. Here, we present a protocol for the cultivation of primary rat inner medullary collecting duct cells suitable for the elucidation of molecular mechanisms underlying AVP-mediated water reabsorption.
