Nucleoside Triphosphate Hydrolases Assay in Toxoplasm gondii and Neospora caninum for High-Throughput Screening using a Robot ArmRiho Kurata *1, Masamitsu Harada *2, Jun Nagai *3, Xiaofeng Cui *4, Takayuki Isagawa 5, Hiroaki Semba 6, Yasuhiro Yoshida 7, Norihiko Takeda 8, Koji Maemura 9, Tomo Yonezawa 3
1Education and Research Center for Pharmaceutical Sciences, Faculty of Pharmacy, Osaka Medical and Pharmaceutical University, 2Independent Scholar, Tokyo, Japan, 3Division of Functional Genomics and Therapeutic Innovation, Research Center for Advanced Genomics, Graduate School of Biomedical Sciences, Nagasaki University, 4School of Chemistry, Chemical Engineering and Life Sciences, School of Materials and Engineering, Wuhan University of Technology, 5Data Science Center, Jichi Medical University, 6Department of Cardiovascular Medicine, The Cardiovascular Institute, 7Department of Immunology and Parasitology, University of Occupational and Environmental Health, 8Division of Cardiology and Metabolism, Center for Molecular Medicine, Jichi Medical University, 9Department of Cardiovascular Medicine, Graduate School of Biomedical Sciences, Nagasaki University Hospital
Toxoplasma gondii and Neospora caninum infections are found in humans and animals and lead to serious health issues. The two parasites share similar nucleoside triphosphate hydrolases and play important roles in propagation and survival. We established a high-standard assay of the enzymes requiring robot arm usage.
