Three-dimensional Inflammatory Human Tissue Equivalents of GingivaLi Xiao 1, Hisashi Okamura 2, Yasuo Kumazawa 2
1Department of Pharmacology, The Nippon Dental University, 2Department of Oral and Maxillofacial Surgery, The Nippon Dental University Hospital
The goal of the protocol is to build an inflammatory human gingiva model in vitro. This tissue model co-cultivates three types of human cells, HaCaT keratinocytes, gingival fibroblasts, and THP-1 macrophages, under three-dimensional conditions. This model can be applied to investigating periodontal diseases, such as gingivitis and periodontitis.
