S'identifier

Shanghai Tech University

2 ARTICLES PUBLISHED IN JoVE

Genetics

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Xuan Yao^1,2, Xing Wang^1,2, Junlai Liu^2,3,4, Linyu Shi¹, Pengyu Huang³, Hui Yang¹

¹Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, ²College of Life Sciences, University of Chinese Academy of Sciences, ³School of Life Science and Technology, Shanghai Tech University, ⁴Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

The clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system provides a promising tool for genetic engineering, and opens up the possibility of targeted integration of transgenes. We describe a homology-mediated end joining (HMEJ)-based strategy for efficient DNA targeted integration in vivo and targeted gene therapies using CRISPR/Cas9.

Chemistry

Synthesis of Persistent Luminescent Nanoparticles for Rewritable Displays and Illumination Applications

Qian Hu¹, Ruotong Li¹, Xingjun Zhu¹

¹School of Physical Science and Technology, Shanghai Tech University

A protocol is presented for the synthesis of persistent luminescent nanomaterials (PLNPs) and their potential applications in rewritable displays and artistic processing utilizing the afterglow effect under ultraviolet light (365 nm) irradiation.