Non-invasive Chromosome Screening (NICS) of Human Preimplantation Embryos: Sample Collection and Chromosomal Ploidy Analysis by MALBAC-NGSRui Fang *1, Yaxin Yao *2, Yiyun Chen 2, Jieliang Ma 2, Li-Yi Cai 1, Sijia Lu *2
1Reproductive Medicine Centre, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical University, 2Department of Clinical Research, Yikon Genomics Co. Ltd.
We report a protocol for chromosome screening of human embryos by using spent culture medium, which avoids embryo biopsy and enables reporting chromosome ploidy using next generation sequencing (NGS). We present the detailed procedure including the preparation of culture medium, whole genome amplification (WGA), NGS library preparation, and data analysis.
