Nanjing University Medical School
3 ARTICLES PUBLISHED IN JoVE
Medicine
Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening
Hui Jiang *1, Yicun Wang *1, Zhantao Deng *1,2,3, Jiewen Jin 2,3, Jia Meng 1, Shuo Chen 1, Jun Wang 1, Yang Qiu 1, Ting Guo 1, Jianning Zhao 1
1Department of Orthopedics, Jinling Hospital, School of Medicine, Nanjing University, 2Center for Translational Medicine, Nanjing University Medical School, 3Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School
This manuscript describes a murine calvarial osteolysis model by exposure to CoCrMo particles, which constitutes an ideal animal model for assessing the interactions between wear particles and various cells in aseptic loosening.
Developmental Biology
Isolation and Culture of Three Kinds of Umbilical Cord Mesenchymal Stem Cells
Yanjun Yang *1, Chunxue Zhang *2, Xiaoqiang Sheng 3
1Department of Obstetrics and Gynecology, the Third Affiliated Hospital, Soochow University, 2Department of Obstetrics and Gynecology, Nanjing First Hospital, Nanjing Medical University, 3Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School
The present protocol describes the isolation and culture of mesenchymal stem cells from the umbilical cord arteries, vein, and Wharton's jelly.
Developmental Biology
Mitochondrial Transfer from Mouse Adipose-Derived Mesenchymal Stem Cells into Aged Mouse Oocytes
Yanjun Yang 1, Chunxue Zhang 2, Xiaoqiang Sheng 3
1Department of Obstetrics and Gynecology, the Third Affiliated Hospital, Soochow University, 2Department of Obstetrics and Gynecology, Nanjing First Hospital, Nanjing Medical University, 3Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School
Here, we describe the isolation of mitochondria from mouse adipose-derived mesenchymal stem cells, and then transfer the mitochondria into aged mouse oocytes to improve the quality of the oocytes.
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