S'identifier

Chinese Academy of Inspection and Quarantine

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Richard W. Loo^1,2, Jane Betty Goh^1,2, Calvin C.H. Cheng¹, Ning Su¹, M. Cynthia Goh^1,2

¹Department of Chemistry, University of Toronto, ²Institute for Optical Sciences, University of Toronto

Simple and reproducible procedures are described for making three structurally distinct collagen assemblies from a common commercially available Type I collagen monomer. Native type, fibrous long spacing or segmental long spacing collagen can be constructed by varying the conditions to which the 300 nm long and 1.4 nm diameter monomer building block is exposed.

Chemistry

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Lesheng Zhang^*1, Tom Cambron^*2, Yueqing Niu¹, Zigang Xu³, Ning Su⁴, Hongyan Zheng⁴, Karl Wei², Paula Ray²

¹Procter and Gamble, Beijing Innovative center, ²Procter and Gamble, Mason Business Center, ³Department of Dermatology, Beijing Children's Hospital, ⁴Chinese Academy of Inspection and Quarantine

Here, we present a protocol for collection of confocal Raman spectra from human subjects in clinical studies combined with chemometric approaches for spectral outlier removal and the subsequent extraction of key features.